产品名称
ECL™ Prime Western Blotting Detection Reagent, Cytiva RPN2236
packaging
pkg of 1 kit
manufacturer/tradename
Cytiva RPN2236
相关类别
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Application
ECL™ Prime Western blotting detection reagent has been used to detect chemiluminescent signals during Western blotting.
Features and Benefits
- Compatible with Rainbow Molecular Weight Markers and Amersham™ ECL DualView™ Western Blotting Markers.
- High signal intensity and sensitivity allows the use of highly diluted primary and secondary antibodies with no reduction in sensitivity.
- Stable signal allows multiple exposures and makes the reagent suitable for large experimental series, allowing convenient handling time between the end of the experiment and detection.
- Optimized for use with Amersham Hybond®-P (PVDF) membranes and compatible with Amersham Hybond-ECL (nitrocellulose) membranes.
- Optimized for imaging with ImageQuant LAS series (CCD-based imaging) and compatible with Amersham Hyperfilm™.
General description
Amersham ECL Prime is a highly sensitive reagent, characterized by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. The high intensity of the emitted signals means that proteins can be detected using highly diluted primary and secondary antibodies, contributing to low background and reducing consumption of reagents.
Amersham ECL Prime consolidates and builds on the benefits of Amersham ECL Plus and Amersham ECL Advance to deliver a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents.
Amersham ECL Prime consolidates and builds on the benefits of Amersham ECL Plus and Amersham ECL Advance to deliver a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents.
Other Notes
The following components are included in the Amersham ECL Prime detection reagent kit.
- RPN2236 Solution A: Luminol solution, 3 × 50 ml Solution B: Peroxide solution, 3 × 50 ml sufficient for 3000 cm2 membrane
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Legal Information
Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
ECL DualView is a trademark of Amersham
Hybond is a registered trademark of Cytiva
Hyperfilm is a trademark of Cytiva
存储类别
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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商品
This page describes troubleshooting strategies in the detection of GST-tagged proteins.
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
描述被分子生物学家用于检测转印膜上蛋白质和核酸的方法的背景和实验方案。
实验方案
This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.
This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.
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