packaging
pack of 1 ea
manufacturer/tradename
Cytiva RPN3688
General description
AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star or ECF detection can be used. Denatured or single-stranded DNA or RNA probe is mixed with the labelling buffer and alkaline phosphatase. The probe is hybridized to the blot using the specially formulated AlkPhos Direct Hybridization buffer, which stabilizes the activity of the enzyme. After a 15-minute pre-hybridization step, hybridization with probe is performed overnight at the required temperature. For higher target amounts, a 2–4 hour hybridization may be sufficient.
Features and Benefits
- Fast and easy to use system suitable for the majority of routine blotting applications, using either DNA, RNA or oligonucleotide probes.
- Alk Phos Direct system has less components than other non-radioactive systems, making it simpler to use as less components need to be removed during the stripping procedure.
- Probe removal is more effective with less damage to the membrane.
- Optimized protocol has been developed capable of labelling probes down to 17 base pairs in length.
- 3–4 hours of time savings compared with conventional, indirect methods are achievable as a result of the rapid and simple labelling reaction.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
AlkPhos Direct
This product is protected by patent numbers US 5,939,257 and EP 770678 owned by Cytiva Bio-Sciences Corp. and equivalent patents and patent applications in other countries.
This product is protected by patent numbers US 5,939,257 and EP 770678 owned by Cytiva Bio-Sciences Corp. and equivalent patents and patent applications in other countries.