form
solid
feature
wetted part: no
packaging
pack of 25 g
manufacturer/tradename
Cytiva RPN6302
color
Yellowish
solubility
cold water: soluble (Easily soluble in cold and hot water)
storage temp.
2-8°C
General description
Used for the efficient alkylation of thiols while minimizing oxidation of competing thiol pairs in protein samples.
Application
Iodoacetamide has been used:
- as a component in urea-sodium dodecyl sulfate (SDS) buffer for yeast cell lysis in immunoprecipitation assay
- in equilibration of pH gradient strips during the second dimension run in isoelectric focusing
- as a protein alkylation agent
Biochem/physiol Actions
Iodoacetamide (IAA) modifies thiol groups in proteins by S-carboxyamidomethylation. Iodoacetamide is an alkylating agent. IAA is commonly used to analyze the key role of cysteine residues in the catalytic reactions of enzymes. It is also used in the derivatization of cysteine residues in proteins for proteomic analysis. IAA irreversibly inhibits glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
Features and Benefits
- Ultrapure, proteomic-grade iodoacetamide is used for efficient alkylation of thiols while minimizing oxidation of the competing thiol pairs in protein samples.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
signalword
Danger
hcodes
存储类别
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
法规信息
新产品
此项目有
COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal
Xu P, et al.
eLife, 6, e28342-e28342 (2017)
Differential effects of iodoacetamide and iodoacetate on glycolysis and glutathione metabolism of cultured astrocytes
Schmidt M and Dringen R
Frontiers in Neuroenergetics, 1(13), 1-1 (2009)
Differential Protein Expression in the Nucleus Accumbens and Amygdala of Lewis and Fischer 344 Rats, and its Relevance in Drug Addiction
Roura-Martinez D, et al.
Journal of drug and alcohol research, 6(3), b1-b8 (2014)
Proteomic analysis of palmitoylated platelet proteins
Dowal L, et al.
Blood, 118(13), e62-e73 (2011)
实验方案
This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.
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