form
solution
usage
sufficient for 25 blots
feature
wetted part: no
manufacturer/tradename
Cytiva RPN810
packaging
pkg of 25 loadings
shipped in
dry ice
storage temp.
−20°C
General description
ECL™ DualVue Western Blotting Markers contain both colored proteins and tagged proteins that introduce convenience and reliability and facilitate accurate size determination of proteins on Western blots.
Application
Amersham™ ECL™ DualVue Western Blotting Markers perform two key functions. First, the prestained marker Proteins confirm Protein transfer in addition to clearly defining blot orientation. Second, the recombinant tagged Proteins are detected on film or by CCD imaging in conjunction with the target Protein(s) of interest, enabling highly accurate molecular mass determination of the target protein(s). The tagged markers are detected easily and specifically by means of a specific conjugate that eliminates any cross contamination between target Protein(s) and markers.
Amersham™ ECL™ DualVue Western Blotting Markers contain a mixture of three prestained colored markers of defined molecular weight (from 15 000 to 100 000) and seven tagged recombinant Proteins (molecular weights from 15 000 to 150 000). Each pack contains ECL™ DualVue markers (125 μL) and S-Protein-HRP conjugate (50 μL), sufficient for 25 standard gel loadings.
Amersham™ ECL™ DualVue Western Blotting Markers contain a mixture of three prestained colored markers of defined molecular weight (from 15 000 to 100 000) and seven tagged recombinant Proteins (molecular weights from 15 000 to 150 000). Each pack contains ECL™ DualVue markers (125 μL) and S-Protein-HRP conjugate (50 μL), sufficient for 25 standard gel loadings.
Features and Benefits
- Detect Proteins on gel and membrane as well as on film.
- Prestained markers indicate efficiency of Protein transfer to membrane and blot orientation.
- Tagged recombinant Protein markers allow accurate calculation of molecular weight of target Protein(s).
- Product is compatible with any HRP substrate as well as both PVDF and nitrocellulose membrane types.
Preparation Note
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
存储类别
12 - Non Combustible Liquids
法规信息
新产品
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John Kerwin et al.
eLife, 9 (2020-04-22)
As part of the Reproducibility Project: Cancer Biology we published a Registered Report (Khan et al., 2015), that described how we intended to replicate selected experiments from the paper "A coding-independent function of gene and pseudogene mRNAs regulates tumour biology"
商品
This page describes troubleshooting strategies in the detection of GST-tagged proteins.
实验方案
This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from Cytiva.
This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.
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