H1404
肝细胞生长因子 人
>95% (SDS-PAGE), recombinant, expressed in Baculovirus infected High-5 cells, lyophilized powder, suitable for cell culture
别名:
HGF, 散射因子, 肝素A
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关于此项目
Biological source:
human
Recombinant:
expressed in Baculovirus infected High-5 cells
Assay:
>95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
~80 kDa
Impurities:
<1.0 EU/μg
产品名称
肝细胞生长因子 人, HGF, recombinant, expressed in Baculovirus infected High-5 cells, suitable for cell culture
biological source
human
Quality Segment
recombinant
expressed in Baculovirus infected High-5 cells
assay
>95% (SDS-PAGE)
form
lyophilized powder
potency
1.0-20.0 ng/mL
quality
endotoxin tested
mol wt
~80 kDa
packaging
pkg of 5x5 μg, pkg of 5 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
<1.0 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... HGF(3082)
Biochem/physiol Actions
肝细胞生长因子（HGF）是肝细胞和多种细胞类型（包括内皮和上皮细胞、黑素细胞和角质形成细胞）的有效促分裂原。它还可介导上皮形态发生。HGF是由met原癌基因编码的酪氨酸激酶受体的配体。
Physical form
从经0.2μm过滤的含有20 mM NaH2PO4 (pH 7.0)和500 mM NaCl,并且每1μg HGF中含50μg BSA的溶液冻干得到
Preparation Note
在含有至少0.1％人或牛血清白蛋白的无菌PBS中以50μg/mL的浓度进行复溶。复溶后，该溶液可以在2-8°C下最多保存一个月，或在-20°C到-70°C下最多保存三个月。请使用手动除霜冷冻柜，并避免反复冻融。
Analysis Note
生物学活性通过其诱导Saos-2人骨肉瘤细胞分泌IL-11的能力进行测定。
Other Notes
NSO表达的重组HGF含有大于95％的异二聚体HGF。昆虫细胞系表达的重组HGF是单链HGF（主要）和一些异二聚体HGF的混合物。单链HGF和异二聚体HGF在 体外 分析中具有相同的活性。
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存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves