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化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-839-5
MDL number:
Specific activity:
≥15 units/mg protein
Biological source:
bacterial (Pseudomonas testosteroni)
biological source
bacterial (Pseudomonas testosteroni)
form
lyophilized solid
specific activity
≥15 units/mg protein
purified by
chromatography
composition
Protein, ≥25%
foreign activity
β-hydroxysteroid dehydrogenase ≤0.5%, alcohol dehydrogenase ≤0.05%
storage temp.
−20°C
Quality Level
Application
来源于假单胞菌 睾丸激素的3α-羟基类固醇脱氢酶已被用于:
- 制备复合电极平台,以用于测定雄甾酮
- 磷酸钾缓冲液中,以用于血清中胆汁酸的生物发光测定
- 通过酶法测定总胆汁盐
Biochem/physiol Actions
3α-羟基类固醇脱氢酶 (3α-HSD) 可以帮助调节雄激素靶组织中雄激素受体 (AR) 的占据。它也可阻碍雄激素的作用。
Physical form
含有磷酸钾缓冲盐、EDTA和DL-二硫苏糖醇的冻干粉末
Other Notes
在β-NAD+存在,pH8.9,25℃条件下,一个单位每分钟可氧化1.0 μmole雄甾酮。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Anne Z Steiner et al.
The Journal of clinical endocrinology and metabolism, 93(4), 1298-1303 (2008-02-07)
Dihydrotestosterone (DHT), the primary active androgen in peripheral target tissues, is metabolized by 3alpha-hydroxysteroid dehydrogenase type III (3alpha-HSD), encoded by the AKR1C2 gene, forming 5alpha-androstane-3alpha,17beta-diol (3alpha-diol). 3alpha-HSD may play a role in the pathogenesis of hirsutism. Our objective was to
Bioluminescent assay for total bile acids in serum with use of bacterial luciferase.
Styrelius I, et al.
Clinical Chemistry, 29(6), 1123-1127 (1983)
Enzyme biosensor for androsterone based on 3alpha-hydroxysteroid dehydrogenase immobilized onto a carbon nanotubes/ionic liquid/NAD+ composite electrode
Mundaca RA, et al.
Talanta, 99(, 697-702 (2012)
Phytosterol ester processing in the small intestine; impact on cholesterol availability for absorption and chylomicron cholesterol incorporation in healthy humans
Amiot MJ, et al.
Journal of Lipid Research, jlr-M013730 (2011)
Laurence Meyer et al.
Neurobiology of disease, 30(1), 30-41 (2008-02-23)
Identification of cellular targets pertinent for the development of effective therapies against pathological pain constitutes a difficult challenge. We combined several approaches to show that 3alpha-hydroxysteroid oxido-reductase (3alpha-HSOR), abundantly expressed in the spinal cord (SC), is a key target, the
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