biological source
human
Quality Segment
recombinant
expressed in E. coli
form
liquid
concentration
1 mg/mL
technique(s)
MALDI-TOF: suitable
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... H3C1(8350)
General description
Histone H3.1 is a histone H3 variant. It differs from H3.2 with residue 96 being a cysteine instead of serine. H3.1 is a replication-dependent histone and is a component of nucleosomes during the S-phase.
Application
Histone H3.1 human has been used in in vitro Schizosaccharomyces pombe Set7 histone methyltransferase assay for matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) studies.
Biochem/physiol Actions
Histone H3.1 human (H3.1) undergoes a majority of post-translational modification especially acetylation and demethylation in lysine 14 and 9 respectively. During differentiation, the levels of H3.1 transcripts decrease as cell division slows down. The incorporation of H3.1 into chromatin is mediated by a chaperone, chromatin assembly factor (CAF-1). A transversion mutation in the H3.1 may be implicated in glioma.
Physical form
Supplied as 1 mg/mL in 20 mM NaPO4, pH 7.0, 0.3 M NaCl, 1 mM EDTA, and 1 mM DTT.
hcodes
存储类别
11 - Combustible Solids
signalword
Warning
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable