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Merck
CN

H2625

血红蛋白 来源于牛血液

suitable for protease substrate, substrate powder

别名:

Hb, 牛血红蛋白, 高铁血红蛋白

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352202
eCl@ss:
42030116
NACRES:
NA.61
MDL number:
Form:
substrate powder
Biological source:
bovine blood
Mol wt:
Mr ~64500
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biological source

bovine blood

Quality Level

form

substrate powder

mol wt

Mr ~64500

technique(s)

activity assay: suitable

solubility

H2O: soluble 20 mg/mL

suitability

suitable for protease substrate

UniProt accession no.

storage temp.

2-8°C

General description

Hemoglobin is the major component of red blood cells and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is a globular protein with α and β chains with every 141 and 146 amino acids respectively. It exists as a tetramer with each monomer having heterocyclic porphyrin ring with iron constituting the heme.

Application

Hemoglobin from bovine blood has been used as a substrate in cathepsin D activity assay. It has also been used to measure the activity of acid proteases (pepsin-like) in the stomach extract.
α-弹性蛋白的溶解度已被应用于弹性蛋白模拟生物材料的构建。

Biochem/physiol Actions

Bovine hemoglobin is used in the production of hemoglobin-vesicles (HbV). It has high thermal stability and high oxygen affinity when compared to human hemoglobin. Bovine hemoglobin interacts with synthetic and azo dyes. Polymerized forms of bovine hemoglobin are used to treat autoimmune hemolytic anemia.
氧气转运蛋白。Fe2+/Fe3+平衡是血液氧合的一种生理指标;脱氧血红蛋白通过将亚硝酸还原为NO来补充反馈回路,而NO是一种可增强血液流向缺氧组织流量的血管扩张剂。
氧转运体,NO 清除剂

Preparation Note

Prepared from washed, lysed and dialyzed erythrocytes.

Disclaimer

由于天然血红蛋白在空气中易于氧化,因此这些制剂主要为高铁血红蛋白。


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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

监管及禁止进口产品

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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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实验方案

This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.

Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.

本方法可用于以血红蛋白为底物的胃蛋白酶活性测定。这是一种分光光度速率终止测定法。


A study of the interaction of bovine hemoglobin with synthetic dyes using spectroscopic techniques and molecular docking
Bansal S, et al.
Frontiers in Chemistry, 4(4), 50-50 (2017)
Luke J Norbury et al.
Biochimie, 93(3), 604-611 (2010-12-21)
Cathepsin proteases are promising vaccine or drug targets for prophylaxis or therapy against Fasciola parasites which express cathepsin L and B proteases during their development. These proteases are believed to be involved in important functions for the parasite, including excystment
High pressure effects on the activities of cathepsins B and D of mackerel and horse mackerel muscle
Fidalgo L, et al.
Czech Journal of Food Sciences, 32(2), 188-193 (2016)



全球贸易项目编号

货号GTIN
H2625-1KG04061826718384
H2625-100G04061838820259
H2625-25G04061835516070