H3034
Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
别名:
Anti-HD3
产品名称
Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 50 kDa
species reactivity
rat, mouse, human
packaging
antibody small pack of 25 μL
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human lymph node sections
immunoprecipitation (IP): 1:2,500 using whole lysate of NIH3T3 cells
microarray: suitable
western blot: 1:10,000 using nuclear extract from HeLa human epithelioid carcinoma cells
western blot: 1:2,000 using whole extract of PC-12 rat pheochromocytoma cell line
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... HDAC3(8841)
mouse ... Hdac3(15183)
rat ... Hdac3(84578)
Application
Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit has been used in:
- immunohistochemistry
- western blotting
- immunoprecipitation
Biochem/physiol Actions
Anti-Histone Deacetylase 3 specifically recognises histone deacetylase 3 by immunoblotting and immunoprecipitation (50 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC3 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC3 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding. The antibody reacts with HDAC3 of human, rat, and mouse origin.
Histone acetylation is a dynamic process whose levels are determined by the net activities of HATs and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Six or seven different mammalian HDACs have been described. HDAC1, HDAC2 and HDAC3 are similar to yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are similar to yeast Hda1 protein. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosomal condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.
Histone deacetylase 3 (HDAC3) acts as a transcriptional modulator of inflammatory genes. It deacetylates lysine residues of histones. HDAC3 associates with nuclear receptor co-repressor (NCoR) or silencing mediator of retinoic and thyroid receptors (SMRTs) and activates the complex. This protein participates in interleukin-1 expression and inflammatory responses.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Histone deacetylase 3 (HDAC3) is an epigenetic modifier and is expressed in various tissues. It belongs to the class I subfamily of histone deacetylases. The HDAC3 gene is mapped on to human chromosome 5q31.3.
Immunogen
synthetic peptide corresponding to amino acids 411-428 of human and chicken HDAC3.
Physical form
Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
常规特殊物品
此项目有
Xiaohong Xiang et al.
Clinical science (London, England : 1979), 136(1), 45-60 (2021-12-18)
Cold storage/rewarming is an inevitable process for kidney transplantation from deceased donors, which correlates closely with renal ischemia-reperfusion injury (IRI) and the occurrence of delayed graft function. Histone deacetylases (HDAC) are important epigenetic regulators, but their involvement in cold storage/rewarming
Histone deacetylase 3 regulates the inflammatory gene expression programme of rheumatoid arthritis fibroblast-like synoviocytes.
Angiolilli C, et al.
Annals of the Rheumatic Diseases, 76(1), 277-285 (2017)
Farah H Bardai et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(29), 11833-11838 (2013-07-19)
Huntington's disease (HD) is caused by a polyglutamine expansion within the huntingtin (Htt) protein. Both loss of function of normal Htt and gain of a toxic function by the polyglutamine-expanded mutant Htt protein have been proposed to be responsible for
Elevated and deregulated expression of HDAC3 in human astrocytic glial tumours
Liby P, et al.
Folia Biologica (Praha.), 52, 21-33 (2006)
Histone Deacetylase-1 (HDAC1) Is a Molecular Switch between Neuronal Survival and Death
Bardai FH, et al.
The Journal of biological chemistry, 287(42), 35444-35453 (2012)
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