Name: Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit
Brand: SIGMA

登录查看公司和协议定价

About This Item

MDL编号:

MFCD02096545

UNSPSC代码:

12352203

NACRES:

NA.41

技术服务

需要帮助？我们经验丰富的科学家团队随时乐意为您服务。

让我们为您提供帮助

生物来源

rabbit

质量水平

200

偶联物

unconjugated

抗体形式

IgG fraction of antiserum

抗体产品类型

primary antibodies

克隆

polyclonal

表单

buffered aqueous solution

分子量

antigen 50 kDa

种属反应性

rat, mouse, human

包装

antibody small pack of 25 μL

技术

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human lymph node sections
immunoprecipitation (IP): 1:2,500 using whole lysate of NIH3T3 cells
microarray: suitable
western blot: 1:10,000 using nuclear extract from HeLa human epithelioid carcinoma cells
western blot: 1:2,000 using whole extract of PC-12 rat pheochromocytoma cell line

UniProt登记号

O15379

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... HDAC3(8841)
mouse ... Hdac3(15183)
rat ... Hdac3(84578)

一般描述

Histone deacetylase 3 (HDAC3) is an epigenetic modifier and is expressed in various tissues. It belongs to the class I subfamily of histone deacetylases. The HDAC3 gene is mapped on to human chromosome 5q31.3.

免疫原

synthetic peptide corresponding to amino acids 411-428 of human and chicken HDAC3.

应用

Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit has been used in:

immunohistochemistry
western blotting
immunoprecipitation

生化/生理作用

Anti-Histone Deacetylase 3 specifically recognises histone deacetylase 3 by immunoblotting and immunoprecipitation (50 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC3 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC3 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding. The antibody reacts with HDAC3 of human, rat, and mouse origin.

Histone acetylation is a dynamic process whose levels are determined by the net activities of HATs and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Six or seven different mammalian HDACs have been described. HDAC1, HDAC2 and HDAC3 are similar to yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are similar to yeast Hda1 protein. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosomal condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.

Histone deacetylase 3 (HDAC3) acts as a transcriptional modulator of inflammatory genes. It deacetylates lysine residues of histones. HDAC3 associates with nuclear receptor co-repressor (NCoR) or silencing mediator of retinoic and thyroid receptors (SMRTs) and activates the complex. This protein participates in interleukin-1 expression and inflammatory responses.

外形

Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

未找到合适的产品？

试试我们的产品选型工具.

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本？

如果您需要特殊版本，可通过批号或批次号查找具体证书。

搜索COA

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持