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H3506

透明质酸酶来源于牛睾丸

Name: 透明质酸酶 来源于牛睾丸
Brand: SIGMA

Type I-S, lyophilized powder, 400-1000 units/mg solid

别名:

玻璃糖醛酸酶, 玻璃酸酶

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关于此项目

化学文摘社编号:

37326-33-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

253-464-3

MDL number:

MFCD00131351

EC 号:

3.2.1.35 (BRENDA, IUBMB)

Specific activity:

400-1000 units/mg solid

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type

Type I-S

Quality Level

200

form

lyophilized powder

specific activity

400-1000 units/mg solid

mol wt

~55 kDa (four subunits of 14 kDa each)

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

UniProt accession no.

Q5E985, Q8SQG8

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

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Application

来自牛睾丸的透明质酸酶已用于乳腺组织、心脏组织和卵母细胞的消化以用于细胞的分离。

适用于制备用于测序的单细胞悬液。

Biochem/physiol Actions

这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-[1→4] 糖苷键。

透明质酸酶可降解透明质酸，并已发现可在癌症进展期间会被不适当地调节。

Other Notes

在 37 ºC、pH 5.35 条件下，一单位每分钟可降解 0.75 μg 多糖透明质酸（通过比浊吸光度测量(λ: 600nm)，与 BSA 复合 45 分钟后测量）。

更多关于用于复合碳水化合物分析的酶的详细信息请见www.sigma-aldrich.com/enzymeexplorer

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

低风险生物材料

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实验方案

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

透明质酸酶的酶法测定 (3.2.1.35)

在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

全球贸易项目编号

货号	GTIN
H3506-1G	04061835557103
H3506-5G	04061835557127
H3506-500MG	04061835557110
H3506-100MG	04061835557097

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