Merck
CN

H3757

Sigma-Aldrich

透明质酸酶 来源于牛睾丸

Type VIII, lyophilized powder, 300-1,000 U/mg

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别名:
玻璃糖醛酸酶, 玻璃酸酶
CAS号:
EC 号:
MDL编号:
NACRES:
NA.54

生物来源

bovine testis

质量水平

偶联物

conjugate (Glucosaminoglycan)

类型

Type VIII

形式

lyophilized powder

比活

300-1,000 U/mg

分子量

~55 kDa (four subunits of 14 kDa each)

UniProt登记号

相关疾病

cancer

储存温度

−20°C

基因信息

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生化/生理作用

这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-[1→4] 糖苷键。
透明质酸酶可降解透明质酸,并已发现可在癌症进展期间会被不适当地调节。

单位定义

在 37 ºC、pH 5.35 条件下,一单位每分钟可降解 0.75 μg 多糖透明质酸(通过比浊吸光度测量(λ: 600nm),与 BSA 复合 45 分钟后测量)。

制备说明

由无菌灌装的I-S型溶液制备。

其他说明

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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示例

T1503
货号
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包装规格/数量

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

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  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How is product H3757, Hyaluronidase from bovine testes, solubilized?

    Product H3757, Hyaluronidase from bovine testes, may be reconstituted at 1 mg/mL in 0.02 M sodium phosphate buffer, pH 7.0 containing 77mM sodium chloride and 0.01% bovine serum albumin.

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商品

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

实验方案

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

在测定透明质酸酶活性时,使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下,一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

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