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H3884

透明质酸酶 来源于牛睾丸

Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid

别名:

玻璃糖醛酸酶, 玻璃酸酶

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关于此项目

化学文摘社编号:
37326-33-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-464-3
MDL number:
MFCD00131351
EC 号:
3.2.1.35 (BRENDA, IUBMB)
Specific activity:
750-3000 units/mg solid
Biological source:
bovine testis
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biological source

bovine testis

Quality Level

200

conjugate

conjugate (Glucosaminoglycan)

type

Type IV-S

form

lyophilized powder (essentially salt-free)

specific activity

750-3000 units/mg solid

mol wt

~55 kDa (four subunits of 14 kDa each)

UniProt accession no.

Q5E985, Q8SQG8

application(s)

diagnostic assay manufacturing

relevant disease(s)

cancer

storage temp.

−20°C

Gene Information

cow ... HYAL1(515397), HYAL2(281838)

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相关类别

Biochem/physiol Actions

这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-[1→4] 糖苷键。
透明质酸酶可降解透明质酸，并已发现可在癌症进展期间会被不适当地调节。

Other Notes

在 37 ºC、pH 5.35 条件下，一单位每分钟可降解 0.75 μg 多糖透明质酸（通过比浊吸光度测量(λ: 600nm)，与 BSA 复合 45 分钟后测量）。
有关复合碳水化合物分析酶的更多信息，请见www.sigma-aldrich.com/enzymeexplorer

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

低风险生物材料

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000521694
0000521693
0000521693
0000521694
0000517507

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实验方案

Enzymatic Assay of Hyaluronidase (3.2.1.35)

To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.

透明质酸酶的酶法测定 (3.2.1.35)

在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。

全球贸易项目编号

货号GTIN
H3884-100MG04061838634924
H3884-1G04061833794173
H3884-50MG04061833794203
H3884-500MG04061833794180

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