应用
组蛋白适用于：
- 作为体外激酶测定的阳性对照，检测Cdc2-细胞周期蛋白B1磷酸化GST-CD-4蛋白的能力
- Cdk5活性测定
- 组蛋白H1激酶测定
- 作为聚ADP-核糖(PAR)受体，检测体外PAR聚合酶活性
- 作为[γ-32P]ATP来源32P的受体，检测小、大黄体细胞来源裂解液的蛋白激酶活性
生化/生理作用
组蛋白是一组DNA结合蛋白，其特征是赖氨酸和精氨酸含量较高。组蛋白的分子量在11-21 kDa之间，取决于具体级分。分离和表征了五种不同的组蛋白级分。H1富含赖氨酸、H2a和H2b略微富含赖氨酸、H3和H4富含精氨酸。H1用于连接染色质链上的各个“念珠”(nu bodies)。
特点和优势
不要将Sigma“类型”名称与Luck及其同事使用的“分级”名称混淆。
制备说明
富含赖氨酸的级分，分离和描述依据文献（de Nooij, E. and Westenbrink, H.G., BiochimBiophys.Acta, 62, 608 (1962)）。
其他说明
经SDS-PAGE鉴定，主要为f1亚型
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
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The real-time changes in viscoelasticity of adsorbed poly(L-lysine) (PLL) and adsorbed histone (lysine rich fraction) due to cross-linking by glutaraldehyde and corresponding release of associated water were investigated using a quartz crystal microbalance with dissipation monitoring (QCM-D) and attenuated total
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