biological source
bovine (calf) thymus
Quality Level
type
Type III-S
form
powder
concentration
≥60% protein (biuret)
technique(s)
activity assay: suitable
solubility
H2O: soluble 10 mg/mL, clear to hazy, colorless to light yellow
storage temp.
2-8°C
Application
组蛋白适用于：
- 作为体外激酶测定的阳性对照，检测Cdc2-细胞周期蛋白B1磷酸化GST-CD-4蛋白的能力
- Cdk5活性测定
- 组蛋白H1激酶测定
- 作为聚ADP-核糖(PAR)受体，检测体外PAR聚合酶活性
- 作为[γ-32P]ATP来源32P的受体，检测小、大黄体细胞来源裂解液的蛋白激酶活性
Biochem/physiol Actions
组蛋白是一组DNA结合蛋白，其特征是赖氨酸和精氨酸含量较高。组蛋白的分子量在11-21 kDa之间，取决于具体级分。分离和表征了五种不同的组蛋白级分。H1富含赖氨酸、H2a和H2b略微富含赖氨酸、H3和H4富含精氨酸。H1用于连接染色质链上的各个“念珠”(nu bodies)。
Features and Benefits
不要将Sigma“类型”名称与Luck及其同事使用的“分级”名称混淆。
Preparation Note
富含赖氨酸的级分，分离和描述依据文献（de Nooij, E. and Westenbrink, H.G., BiochimBiophys.Acta, 62, 608 (1962)）。
Other Notes
经SDS-PAGE鉴定，主要为f1亚型
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
监管及禁止进口产品
此项目有
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| H5505-250MG | 04061833271513 |
| H5505-25MG | 04061833799475 |
| H5505-100MG | 04061833271506 |