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Merck
CN

H6024

Hoechst染色 溶液

别名:

双苯甲亚胺 H 33258

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关于此项目

化学文摘社编号:
UNSPSC Code:
12352207
PubChem Substance ID:
MDL number:
Form:
solution
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form

solution

application(s)

histology

storage temp.

2-8°C

SMILES string

O.Cl.Cl.Cl.CN1CCN(CC1)c2ccc3NC(=NCc3c2)c4ccc5NC(=NCc5c4)c6ccc(O)cc6

InChI

1S/C27H28N6O.3ClH.H2O/c1-32-10-12-33(13-11-32)22-5-9-25-21(15-22)17-29-27(31-25)19-4-8-24-20(14-19)16-28-26(30-24)18-2-6-23(34)7-3-18;;;;/h2-9,14-15,34H,10-13,16-17H2,1H3,(H,28,30)(H,29,31);3*1H;1H2

InChI key

OWRSPPSBNWJJAR-UHFFFAOYSA-N

General description

Hoechst染色是蓝色荧光染色家族的一分子,通常用于DNA染色。它能够以生物合成方式,掺入固定的细胞制备和不固定的培养细胞中的间期细胞核。它可以被用来检测5-溴脱氧尿苷生物合成掺入活细胞DNA。Hoechst染色的特性使得荧光激活细胞和染色体在某种程度上按照DNA合成排列,这样就在生物化学和DNA复制相关细胞学分析中间架起了桥梁。

Application

是一种可以用于支原体染色的荧光DNA染色。

Other Notes

含有 0.5μg/ml Hoechst 双苯甲亚胺33258氟铬染色和硫柳汞。


存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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历史批次信息供参考:

分析证书(COA)

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Marisa Müller et al.
PLoS biology, 9(4), e1000611-e1000611 (2011-04-29)
In eukaryotes, hundreds of mRNAs are localized by specialized transport complexes. For localization, transcripts are recognized by RNA-binding proteins and incorporated into motor-containing messenger ribonucleoprotein particles (mRNPs). To date, the molecular assembly of such mRNPs is not well understood and
S A Latt et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 23(7), 493-505 (1975-07-01)
A number of applications of the detection of deoxyribonucleic acid synthesis by fluorescence microscopy are illustrated. These include (a) the analysis of sister chromatid exchanges and sister chromatid segregation at mitosis, (b) the location of chromosome regions containing deoxyribonucleic acid
Nicola Cirillo et al.
British journal of cancer, 117(7), 984-993 (2017-08-11)
Recent studies have shown that production of cortisol not only takes place in several non-adrenal peripheral tissues such as epithelial cells but, also, the local inter-conversion between cortisone and cortisol is regulated by the 11β-hydroxysteroid dehydrogenases (11β-HSDs). However, little is