H6254
透明质酸酶 来源于绵羊睾丸
Type V, lyophilized powder, ≥1,500 units/mg solid
别名:
玻璃糖醛酸酶, 玻璃酸酶
产品名称
透明质酸酶 来源于绵羊睾丸, Type V, lyophilized powder, ≥1,500 units/mg solid
biological source
sheep testis
type
Type V
form
lyophilized powder
specific activity
≥1,500 units/mg solid
mol wt
55 kDa
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
Quality Level
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Application
透明质酸酶已用于：
- 用作从生精小管中分离睾丸和生殖细胞的酶混合物组分
- 预处理脱蜡腺瘤切片，以进行免疫组织化学分析
- 酶解脐带血管，从而产生脐带间质干细胞系
Biochem/physiol Actions
商业领域采用绵羊睾丸来源透明质酸酶分别解聚结缔组织和软骨中的透明质酸和硫酸软骨素。
这些酶可随机裂解透明质酸、软骨素和硫酸软骨素中的 β-N-乙酰己糖胺-(1→4) 糖苷键。
General description
透明质酸酶有助于哺乳动物卵子受精，因此在睾丸中的含量很高。哺乳动物透明质酸酶具有催化表皮生长因子（EGF）样结构域和富含C端半胱氨酸的区域。
Other Notes
在 37 ºC、pH 5.35 条件下，一单位每分钟可降解 0.75 μg 多糖透明质酸（通过比浊吸光度测量(λ: 600nm)，与 BSA 复合 45 分钟后测量）。
查看更多用于复合糖分析的酶的信息，请访问 www.sigma-aldrich.com/enzymeexplorer
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
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商品
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
实验方案
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
在测定透明质酸酶活性时，使用了一种在600 nm处的比浊测定法进行测定。在pH 5.35、37 °C下，一单位的透明质酸酶活性可每分钟引起0.330的吸光值改变。
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