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H7033

Sigma-Aldrich

PerfectHyb™ Plus杂交缓冲液

for Northern and Southern blotting, solution

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别名:
hyb buffer, hyb solution, hybridization solution, northern hybridization buffer, southern hybridization buffer
UNSPSC代码:
12161700
NACRES:
NA.25

一般描述

Sigma的PerfectHyb Plus是一种即用型杂交缓冲液，适用于Northern和Southern印迹方案。 使用PerfectHyb Plus可将杂交时间缩短至两小时。

应用

可用作Northern印迹、Southern印迹、菌落/噬斑印迹和斑点印迹中所使用的杂交缓冲液。

特点和优势

  • 快速且易于使用
  • 灵敏度比传统缓冲液高20倍
  • 不需要封闭步骤
  • 与中性和带正电的膜兼容
  • 与化学发光、比色和DIG检测分析兼容
  • 兼容放射性和非放射性探针
  • 与单链或双链DNA、RNA和寡核苷酸探针兼容

其他说明

在溶液中形成的少量沉淀物可以在30-35℃下，通过偶尔轻缓颠倒进行溶解。

法律信息

PerfectHyb is a trademark of Sigma-Aldrich Co. LLC

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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Shinpei Kawaoka et al.
RNA (New York, N.Y.), 15(7), 1258-1264 (2009-05-23)
Genetic studies and large-scale sequencing experiments have revealed that the PIWI subfamily proteins and PIWI-interacting RNAs (piRNAs) play an important role in germ line development and transposon control. Biochemical studies in vitro have greatly contributed to the understanding of small
Hada Wuriyanghan et al.
PloS one, 6(11), e27736-e27736 (2011-11-24)
The potato/tomato psyllid, Bactericerca cockerelli (B. cockerelli), and the Asian citrus psyllid, Diaphorina citri (D. citri), are very important plant pests, but they are also vectors of phloem-limited bacteria that are associated with two devastating plant diseases. B. cockerelli is
Xianfeng Morgan Xu et al.
The Plant cell, 19(5), 1537-1548 (2007-05-22)
Vertebrate Tpr and its yeast homologs Mlp1/Mlp2, long coiled-coil proteins of nuclear pore inner basket filaments, are involved in mRNA export, telomere organization, spindle pole assembly, and unspliced RNA retention. We identified Arabidopsis thaliana NUCLEAR PORE ANCHOR (NUA) encoding a
Jonathan C Lamb et al.
Genetics, 173(2), 1007-1021 (2006-04-04)
Divergence of abundant genomic elements among the Zea and Tripsacum genera was examined cytologically and a tool kit established for subsequent studies. The LTR regions from the CRM, Huck, Grande, Prem1, Prem2/Ji, Opie, Cinful-1, and Tekay retroelement families were used
Sivaramakrishnan Muthuswamy et al.
Planta, 233(1), 201-208 (2010-09-28)
Protein sumoylation plays an important role in plant development, flowering-time regulation, and abiotic stress response. However, the molecular role of sumoylation in these pathways is largely unknown. It was shown previously that in mutants of the inner nuclear basket nucleoporin
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实验方案

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern和Southern印迹实验方案及简介

Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

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