H7033
PerfectHyb™ Plus杂交缓冲液
for Northern and Southern blotting, solution
别名:
hyb buffer, hyb solution, hybridization solution, northern hybridization buffer, southern hybridization buffer
一般描述
Sigma的PerfectHyb Plus是一种即用型杂交缓冲液，适用于Northern和Southern印迹方案。 使用PerfectHyb Plus可将杂交时间缩短至两小时。
应用
可用作Northern印迹、Southern印迹、菌落/噬斑印迹和斑点印迹中所使用的杂交缓冲液。
特点和优势
- 快速且易于使用
- 灵敏度比传统缓冲液高20倍
- 不需要封闭步骤
- 与中性和带正电的膜兼容
- 与化学发光、比色和DIG检测分析兼容
- 兼容放射性和非放射性探针
- 与单链或双链DNA、RNA和寡核苷酸探针兼容
其他说明
在溶液中形成的少量沉淀物可以在30-35℃下，通过偶尔轻缓颠倒进行溶解。
法律信息
PerfectHyb is a trademark of Sigma-Aldrich Co. LLC
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Shinpei Kawaoka et al.
RNA (New York, N.Y.), 15(7), 1258-1264 (2009-05-23)
Genetic studies and large-scale sequencing experiments have revealed that the PIWI subfamily proteins and PIWI-interacting RNAs (piRNAs) play an important role in germ line development and transposon control. Biochemical studies in vitro have greatly contributed to the understanding of small
Xianfeng Morgan Xu et al.
The Plant cell, 19(5), 1537-1548 (2007-05-22)
Vertebrate Tpr and its yeast homologs Mlp1/Mlp2, long coiled-coil proteins of nuclear pore inner basket filaments, are involved in mRNA export, telomere organization, spindle pole assembly, and unspliced RNA retention. We identified Arabidopsis thaliana NUCLEAR PORE ANCHOR (NUA) encoding a
Sivaramakrishnan Muthuswamy et al.
Planta, 233(1), 201-208 (2010-09-28)
Protein sumoylation plays an important role in plant development, flowering-time regulation, and abiotic stress response. However, the molecular role of sumoylation in these pathways is largely unknown. It was shown previously that in mutants of the inner nuclear basket nucleoporin
Edyta Koscianska et al.
TheScientificWorldJournal, 11, 102-117 (2011-01-11)
This protocol describes how to perform northern blot analyses to detect microRNAs and their precursors with single-nucleotide resolution, which is crucial for analyzing individual length variants and for evaluating relative quantities of unique microRNAs in cells. Northern blot analysis consists
Alavala Matta Reddy et al.
BMC genomics, 10, 65-65 (2009-02-07)
MicroRNAs (miRNAs) are small ~22-nt regulatory RNAs that can silence target genes, by blocking their protein production or degrading the mRNAs. Pig is an important animal in the agriculture industry because of its utility in the meat production. Besides, pig
实验方案
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持