Anti-HtrA2 (C-terminal) antibody produced in rabbit

High temperature requirement protein A2 (HtrA2) or serine peptidase 2 belongs to the HtrA family of serine proteases. It is a mitochondrial serine protease, that comprises of an N-terminal mitochondrial localization sequence (MLS), an inhibitor of apoptosis protein (IAP) binding domain (IBM) and a single C-terminal PDZ domain. It also has a transmembrane and a serine protease domain. HTRA2 gene is mapped to human chromosome 2p13.1.

Anti-HtrA2 (C-terminal) antibody produced in rabbit may been used in immunoblotting.

Anti-HtrA2 (C-terminal) specifically recognizes human and rat HtrA2.

High temperature requirement protein A2 (HtrA2) exhibits endoproteolytic activity by cleaving misfolded proteins and other cellular proteins. It is essential for mitochondrial homeostasis and regulates apoptosis through multiple pathways, including caspase-dependent and caspase-independent cell death. Mature HtrA2, via its IAP binding domain favors the neutralizing inhibitor of apoptosis proteins (IAPs) by promoting the cytochrome c-dependent caspase activation. Mutations that inactivate HtrA2 gene are associated with neurodegenerative diseases such as Parkinson′s disease and Alzheimer′s disease. Mutation in the HtrA2 gene is also implicated in the early-onset mitochondrial syndromes including the 3-methylglutaconic aciduria. HtrA2 variants are responsible infantile neurodegeneration, mitochondrial dysfunction and promote apoptosis.

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

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