shelf life
2 yr at 2‑8 °C
Quality Level
packaging
pkg of 10 ea, pkg of 50 ea
pore size
100 nm pore size
capacity
>0.5 mg/column
storage temp.
2-8°C
General description
试剂盒含有针对每种离心柱的3种组合管。
Application
预填充的即用型HIS-Select离心柱可实现对含有组氨酸标签蛋白的小规格细胞粗提取物进行快速而一致的纯化。该离心柱包含二氧化硅颗粒基质并使用HIS-Select专利的不带电荷的亲水性镍螯合键合化学。HIS-Select离心柱对组氨酸标记的蛋白具有高度选择性，而对其他蛋白的结合特异性极低。这些便捷的离心柱可实现快速的一步法纯化，在不到15分钟的时间内分离出至少150 μg的组氨酸标签蛋白。
Features and Benefits
- 高选择性确保了高纯度
- 便捷且即用
- 每次使用的一致性蛋白结合
- 兼容CelLytic™细胞裂解试剂
Legal Information
CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Maria A Schumacher et al.
Nucleic acids research, 46(14), 7405-7417 (2018-06-16)
Streptomyces are filamentous bacteria with a complex developmental life cycle characterized by the formation of spore-forming aerial hyphae. Transcription of the chaplin and rodlin genes, which are essential for aerial hyphae production, is directed by the extracytoplasmic function (ECF) σ
Ozan Gundogdu et al.
Journal of bacteriology, 193(16), 4238-4249 (2011-06-07)
Campylobacter jejuni is the leading bacterial cause of human gastroenteritis worldwide. Despite stringent microaerobic growth requirements, C. jejuni is ubiquitous in the aerobic environment and so must possess regulatory systems to sense and adapt to external stimuli, such as oxidative
Anne Durand et al.
The Journal of biological chemistry, 281(40), 29558-29567 (2006-07-26)
In Escherichia coli, the ammonia channel AmtB and the P(II) signal transduction protein GlnK constitute an ammonium sensory system that effectively couples the intracellular nitrogen regulation system to external changes in ammonium availability. Binding of GlnK to AmtB apparently inactivates
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| H7787-50EA | 04061833804759 |
| H7787-10EA | 04061835573271 |