H8163
Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
别名:
Anti-HDAC5
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关于此项目
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, ICC, IHC, IP, WB CL
Citations:
9
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~124 kDa
species reactivity
mouse, human, rat
technique(s)
immunocytochemistry: 1-2 μg/mL using cultured NIH-3T3 or HeLa cells, immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin-embedded human breast carcinoma section, immunoprecipitation (IP): 1-2 μg using extract of 293T cells expressing recombinant mouse HDAC5, microarray: suitable, western blot (chemiluminescent): 2-4 μg/mL using whole extract of human embryonal kidney 293T cells and whole extract of rat pheochromocytoma PC-12 cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HDAC5(10014)
mouse ... Hdac5(15184)
rat ... Hdac5(84580)
General description
Histone Deacetylase 5 (HDAC5) belongs to the class IIa HDAC family. The localization of HDAC5 is both nuclear and cytoplasmic. HDAC5 gene is mapped to human chromosome at 17q21.31.
Immunogen
synthetic peptide corresponding to amino acid residues 384-399 of human HDAC5 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in rat and mouse.
Application
Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit has been used in:
- chromatin immunoprecipitation
- immunostaining
- immunocytochemistry
- immunoprecipitation
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Chromatin immunoprecipitation (1 paper)
Biochem/physiol Actions
Anti-Histone Deacetylase 5 (HDAC5)(NA-16) recognizes human, rat, and mouse HDAC5.
Histone Deacetylase 5 (HDAC5) shuttling to the cytoplasm occurs during myogenic differentiation, the nuclear export being stimulated by calcium/ calmodulin-dependent protein kinase (CaMK) phosphorylation at Ser259 and Ser498. HDAC5 activity is important for the differentiation of muscle cells by binding, through its N-terminal domain, to the myocyte enhancer factor-2 (MEF2) protein, thus repressing expression of MEF2 downstream genes.Overexpression of HDAC5 in various cancer cells suppresses their growth by induction of apoptosis in a p53-independent manner. It also plays a key role in many biological processes including the synoviocyte activation, neural regeneration and repair. HDAC5 is highly expressed in breast cancer and contributes to tumor progression.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk
nwg
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Stress-induced epigenetic transcriptional memory of acetylcholinesterase by HDAC4
Sailaja BS, et al.
Proceedings of the National Academy of Sciences, 109(52), E3687-E3695 (2012)
Targeting histone deacetylases for cancer therapy: from molecular mechanisms to clinical implications
Li Z and Zhu WG
International Journal of Biological Sciences, 10(7), 757-757 (2014)
Functional interaction of histone deacetylase 5 (HDAC5) and lysine-specific demethylase 1 (LSD1) promotes breast cancer progression
Cao C, et al.
Oncogene, 36(1), 133-145 (2017)
HDAC5, a potential therapeutic target and prognostic biomarker, promotes proliferation, invasion and migration in human breast cancer
Li A, et al.
Testing, 7(25), 37966-37966 (2016)
HDAC5 is a novel injury-regulated tubulin deacetylase controlling axon regeneration
Cho Y and Cavalli V
The Embo Journal, 31(14), 3063-3078 (2012)
相关内容
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