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Merck
CN

H8280

N-羟基琥珀酰亚胺基琼脂糖凝胶 4 快 慢

matrix fast-flow highly cross-linked 4% beaded agarose

别名:

N -羟基琥珀酰亚胺-琼脂糖

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UNSPSC Code:
23151817
NACRES:
NA.56
MDL number:
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form

isopropanol suspension

extent of labeling

16-23 μmol per mL

technique(s)

protein array: suitable

matrix

fast-flow highly cross-linked 4% beaded agarose

matrix active group

N-hydroxysuccinimide active ester

matrix spacer

6 atoms (when ligands are coupled by displacement of NHS)

storage temp.

2-8°C

Quality Level

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General description

N-羟基琥珀酰-琼脂糖 4 Fast Flow是一种交联预活化的基质,通过间隔臂将Sepharose 4 Fast Flow与6-氨基己酸混合获得。与N-羟基琥珀酰的酯化反应激活了末端羧基。含有配体的基直接与该活性酯相互作用,形成化学性质非常稳定的酰胺键。

Application

N-羟基琥珀酰-琼脂糖 4 Fast Flow已被用作基于磁颗粒的视觉检测系统的一部分,以实现扩增基因结合后的分离。它也被用来固定蛋白质在拉下试验。

Features and Benefits

  • 特别推荐用于工艺规模应用中含氨基的小蛋白和多肽的偶联
  • 高水平的预激活,这提供了所选配体的高度替代
  • NHS偶联方法使配体的化学稳定附着

Legal Information

Sepharose is a trademark of Cytiva

pictograms

FlameExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

3 - Flammable liquids

wgk

WGK 1

flash_point_f

53.6 °F

flash_point_c

12 °C

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

危险化学品
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Seulggie Choi et al.
Molecular immunology, 48(15-16), 2207-2213 (2011-07-12)
Porphyromonas gingivalis causes periodontal diseases and its lipopolysaccharide (LPS) is considered as a major virulence factor responsible for pathogenesis. Since initial recognition of P. gingivalis LPS (Pg.LPS) in the oral cavity might be crucial for the host response, we identified
Yoo Min Park et al.
Biosensors & bioelectronics, 141, 111415-111415 (2019-06-16)
We focused on the development of a hand-held pathogen-detection device using smartphone-embedded electronic elements combined with functionalized magnetic particles (MPs) and sepharose. To perform affinity chromatography for evaluating DNA amplicons, avidin-conjugated MPs and succinimide-linked sepharose were used with biotin-primers. To
Woo-Hyeon Byeon et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(2), 361-363 (2004-05-12)
Combinatorial phage display was used to discover peptides that selectively bind to the alpha-cobratoxin (neurotoxin) component of the multi-component venom of the Thai cobra, Naja kaouthia. Peptide sequences determined in this way were synthesized chemically and were covalently attached to
Yoo Min Park et al.
Talanta, 195, 97-102 (2019-01-11)
The current study focuses on developing a system for visually detecting an amplified bacterial (Escherichia coli O157:H7) gene using a heavy metal particle (MP) and functionalized porous sepharose gel. To functionalize DNA-specificity to the MP, an avidin-modified MP was employed
Julia V Hartig et al.
Nucleic acids research, 39(9), 3836-3851 (2011-01-20)
In Drosophila, siRNAs are classified as endo- or exo-siRNAs based on their origin. Both are processed from double-stranded RNA precursors by Dcr-2 and then loaded into the Argonaute protein Ago2. While exo-siRNAs serve to defend the cell against viruses, endo-siRNAs

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