recombinant
expressed in E. coli
Quality Segment
assay
≥95% (SDS-PAGE)
mol wt
22 kDa (plus 114 kDa galactosidse)
concentration
~1 mg/mL
shipped in
dry ice
storage temp.
−20°C
Gene Information
hepatitis C virus ... HCVgp1(951475)
human ... HBVgp4(944568)
General description
Hepatitis C virus core antigen (HCV-cAg) is a well-preserved antigenic protein of the internal capsid.
Hepatitis C virus glycoprotein 1 (HCVgp1), also referred to as polyprotein, is crucial for the induction of double-membrane vesicles, sites of HCV RNA amplification. The amino terminal of polyprotein contains the structural proteins core, envelope protein 1 (E1) and E2. The proteolytic cleavage of polyprotein results in the formation of 10 mature viral proteins which are needed for viral replication and assembly.
amino acids 2-192 of HCV polyprotein
Application
Hepatitis core antigen (HCA) has been used as an analyte in enzyme-linked immunosorbent assay (ELISA) to study the mechanism of bovine serum albumin (BSA) blocking in common ELISA protocols.
Biochem/physiol Actions
Hepatitis C virus core antigen (HCV-cAg) may be a less expensive way to diagnose active hepatitis C virus (HCV) infection in the hemodialysis (HD) population.
Positive control for HCV antibodies.
Reacts strongly with human HCV positive serum.
Physical form
Solution in 8 M Urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol.
Other Notes
HCV core antigen [2-192]-Galactosidase-tagged at the N-terminus.
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable