H9037
抗-HA−TRITC 抗体，小鼠单克隆 小鼠抗
~1 mg/mL, clone HA-7, purified from hybridoma cell culture
别名:
单克隆抗-HA, 抗-HA, 抗-流感血凝素
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关于此项目
Conjugate:
TRITC conjugate
Clone:
HA-7, monoclonal
Application:
IF (d)
Citations:
9
biological source
mouse
conjugate
TRITC conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HA-7, monoclonal
form
buffered aqueous solution
storage condition
protect from light
concentration
~1 mg/mL
technique(s)
direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
Quality Level
General description
单克隆抗-HA来源于小鼠骨髓瘤细胞和BALB/c小鼠脾细胞融合产生的HA-7杂交瘤。单克隆抗HA通过免疫细胞化学识别HA标记的融合蛋白上的HA标签序列。该抗体与N-端和C-端HA标签融合蛋白特异反应。
Immunogen
与人流感病毒血凝素(HA)片段相对应的合成肽，称为HA-标签，与KLH结合
Application
单克隆抗HA-TRITC抗体用于测试DOT/ICM效应子SDHA是否对于大蜡螟和A/J小鼠中嗜肺军团菌的毒力是必需的。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
免疫荧光分析（1篇论文）
免疫荧光分析（1篇论文）
Biochem/physiol Actions
该抗体可识别HA-标签蛋白的天然和还原变性形式，并且可与 大肠杆菌 或哺乳动物表达的N-末端或C-末端HA-标签融合蛋白发生反应。
Physical form
溶于含有1%牛血清白蛋白和15mM叠氮化钠（作为防腐剂）的0.01M磷酸盐缓冲生理盐水（pH 7.4）中的溶液。
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
低风险生物材料
常规特殊物品
此项目有
Yasuhiro Yamazaki et al.
Science immunology, 5(44) (2020-03-01)
We investigated the molecular and cellular basis of severe combined immunodeficiency (SCID) in six patients with otofaciocervical syndrome type 2 who failed to attain T cell reconstitution after allogeneic hematopoietic stem cell transplantation, despite successful engraftment in three of them.
D J Scott et al.
British journal of urology, 58(2), 119-124 (1986-04-01)
To assess the effectiveness of the surgical correction of vesico-ureteric reflux, current renal function was determined in 56 children with scarred kidneys who had undergone ureteric reimplantation between 1978 and 1983. The children were aged between 2 and 15 years
P O Olins et al.
Current opinion in biotechnology, 4(5), 520-525 (1993-10-01)
Recent advances in protein expression in E. coli have focused primarily on the enhancement of protein quality. Problems in mRNA translation such as inefficient initiation, mistranslation, frame-shifting and frame-hopping can often be addressed by altering heterologous gene-coding sequences. Fusion technology
Clare R Harding et al.
Infection and immunity, 81(7), 2598-2605 (2013-05-08)
Legionella pneumophila is an intracellular bacterium that resides within amoebae and macrophages in a specialized compartment termed the Legionella-containing vacuole (LCV). As well as providing an intracellular niche for replication, the LCV helps to prevent the release of bacterial components
Gunnar N Schroeder et al.
Infection and immunity, 83(10), 3989-4002 (2015-07-29)
Legionella pneumophila is a bacterial pathogen that thrives in alveolar macrophages, causing a severe pneumonia. The virulence of L. pneumophila depends on its Dot/Icm type IV secretion system (T4SS), which delivers more than 300 effector proteins into the host, where
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