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Merck
CN

H9394

汉克斯平衡盐 溶液

Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

别名:

HBSS

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关于此项目

NACRES:
NA.75
UNSPSC Code:
12352207

主要文件

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产品名称

汉克斯平衡盐 溶液, Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L

shipped in

ambient

Quality Level

400

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Application

Hanks′平衡盐溶液已用于：
  • 作为小鼠皮层切片培养基组分
  • 作为解离缓冲液组分，用于人诱导多能干细胞(hiPSCs)和hiPSC来源心肌细胞(CMs)培养
  • 小鼠肝脏的原位灌注

Biochem/physiol Actions

平衡盐溶液在细胞培养中起多种作用，包括作为冲洗、运输和稀释用液以维持细胞内外的渗透压平衡，供给细胞正常代谢所需的水和大量无机离子。还参与提供缓冲体系，维持培养基的生理pH范围(7.2-7.6)，提供细胞代谢所需的主要能量。

General description

Hanks平衡盐溶液推荐用于悬浮培养或细胞结块时。

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
RNBM0561
RNBL8854
RNBL5851
RNBL4924
RNBL4477

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Nature communications, 10(1), 281-281 (2019-01-19)
Macrophages serve multiple functions including immune regulation, morphogenesis, tissue homeostasis and healing reactions. The current paradigm holds that mammary gland macrophages first arise postnatally during the prepubertal period from the bone marrow-derived monocytes. Here we delineate the origins of tissue-resident
Sunny Nigam et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 36(3), 670-684 (2016-01-23)
The performance of complex networks, like the brain, depends on how effectively their elements communicate. Despite the importance of communication, it is virtually unknown how information is transferred in local cortical networks, consisting of hundreds of closely spaced neurons. To
Adrian Westhaus et al.
Human gene therapy, 31(9-10), 575-589 (2020-02-01)
Adeno-associated virus (AAV) vectors are quickly becoming the vectors of choice for therapeutic gene delivery. To date, hundreds of natural isolates and bioengineered variants have been reported. While factors such as high production titer and low immunoreactivity are important to
Pratyusha Mandal et al.
Viruses, 12(11) (2020-11-01)
Cytomegaloviruses all encode the viral inhibitor of caspase-8-induced apoptosis (vICA). After binding to this initiator caspase, vICA blocks caspase-8 proteolytic activity and ability to activate caspase-3 and/or caspase-7. In this manner, vICA has long been known to prevent apoptosis triggered
Sarah Pedretti et al.
PloS one, 14(6), e0218432-e0218432 (2019-06-21)
High density lipoprotein (HDL) protects against myocardial infarction via mechanisms that remain unclear. STAT3 (signal transducer and activator of transcription 3) plays a key role in HDL-induced cardioprotection. In the heart, microRNAs (miRNAs) are involved in ischemia reperfusion injury. We
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实验方案

Trypsin Cell Dissociation Protocol

Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.

StableCell™ Trypsin Solutions

StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.

用胰蛋白酶从培养表面去除贴壁细胞

胰蛋白酶可用于从培养表面去除贴壁细胞。从培养基底物除去细胞的最常用方法是用胰蛋白酶或胰蛋白酶/EDTA溶液处理。

StableCell™胰蛋白酶溶液

StableCell™胰蛋白酶溶液设计用于执行标准胰蛋白酶溶液所实现的细胞解离，但不同的是前者无需等分、冷冻和解冻。这样可以节省大量时间。

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