H9394
汉克斯平衡盐 溶液
Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture
别名:
HBSS
质量水平
无菌性
sterile-filtered
表单
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L
运输
ambient
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一般描述
Hanks平衡盐溶液推荐用于悬浮培养或细胞结块时。
应用
Hanks′平衡盐溶液已用于：
- 作为小鼠皮层切片培养基组分
- 作为解离缓冲液组分，用于人诱导多能干细胞(hiPSCs)和hiPSC来源心肌细胞(CMs)培养
- 小鼠肝脏的原位灌注
生化/生理作用
平衡盐溶液在细胞培养中起多种作用，包括作为冲洗、运输和稀释用液以维持细胞内外的渗透压平衡，供给细胞正常代谢所需的水和大量无机离子。还参与提供缓冲体系，维持培养基的生理pH范围(7.2-7.6)，提供细胞代谢所需的主要能量。
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Annemiek A van Berkel et al.
Journal of neurochemistry, 157(3), 450-466 (2020-12-02)
Loss of the exocytic Sec1/MUNC18 protein MUNC18-1 or its target-SNARE partners SNAP25 and syntaxin-1 results in rapid, cell-autonomous and unexplained neurodegeneration, which is independent of their known role in synaptic vesicle exocytosis. cis-Golgi abnormalities are the earliest cellular phenotypes before
Norma Jäppinen et al.
Nature communications, 10(1), 281-281 (2019-01-19)
Macrophages serve multiple functions including immune regulation, morphogenesis, tissue homeostasis and healing reactions. The current paradigm holds that mammary gland macrophages first arise postnatally during the prepubertal period from the bone marrow-derived monocytes. Here we delineate the origins of tissue-resident
Adrian Westhaus et al.
Human gene therapy, 31(9-10), 575-589 (2020-02-01)
Adeno-associated virus (AAV) vectors are quickly becoming the vectors of choice for therapeutic gene delivery. To date, hundreds of natural isolates and bioengineered variants have been reported. While factors such as high production titer and low immunoreactivity are important to
Simon Joost et al.
Cell reports, 25(3), 585-597 (2018-10-18)
Epithelial tissues, such as the skin, rely on cellular plasticity of stem cells (SCs) from different niches to restore tissue function after injury. How these molecularly and functionally diverse SC populations respond to injury remains elusive. Here, we genetically labeled
Sarah Pedretti et al.
PloS one, 14(6), e0218432-e0218432 (2019-06-21)
High density lipoprotein (HDL) protects against myocardial infarction via mechanisms that remain unclear. STAT3 (signal transducer and activator of transcription 3) plays a key role in HDL-induced cardioprotection. In the heart, microRNAs (miRNAs) are involved in ischemia reperfusion injury. We
实验方案
胰蛋白酶可用于从培养表面去除贴壁细胞。从培养基底物除去细胞的最常用方法是用胰蛋白酶或胰蛋白酶/EDTA溶液处理。
StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.
StableCell™胰蛋白酶溶液设计用于执行标准胰蛋白酶溶液所实现的细胞解离，但不同的是前者无需等分、冷冻和解冻。这样可以节省大量时间。
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