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Merck
CN

H9394

Sigma-Aldrich

汉克斯平衡盐 溶液

Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

别名:

HBSS

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关于此项目

UNSPSC代码:
12352207
NACRES:
NA.75

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质量水平

400

无菌性

sterile-filtered

表单

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L

运输

ambient

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相关类别

一般描述

Hanks平衡盐溶液推荐用于悬浮培养或细胞结块时。

应用

Hanks′平衡盐溶液已用于：
  • 作为小鼠皮层切片培养基组分
  • 作为解离缓冲液组分，用于人诱导多能干细胞(hiPSCs)和hiPSC来源心肌细胞(CMs)培养
  • 小鼠肝脏的原位灌注

生化/生理作用

平衡盐溶液在细胞培养中起多种作用，包括作为冲洗、运输和稀释用液以维持细胞内外的渗透压平衡，供给细胞正常代谢所需的水和大量无机离子。还参与提供缓冲体系，维持培养基的生理pH范围(7.2-7.6)，提供细胞代谢所需的主要能量。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
RNBM0561
RNBL8854
RNBL5851
RNBL4924
RNBL4477

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Human gene therapy, 31(9-10), 575-589 (2020-02-01)
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Simon Joost et al.
Cell reports, 25(3), 585-597 (2018-10-18)
Epithelial tissues, such as the skin, rely on cellular plasticity of stem cells (SCs) from different niches to restore tissue function after injury. How these molecularly and functionally diverse SC populations respond to injury remains elusive. Here, we genetically labeled
Sarah Pedretti et al.
PloS one, 14(6), e0218432-e0218432 (2019-06-21)
High density lipoprotein (HDL) protects against myocardial infarction via mechanisms that remain unclear. STAT3 (signal transducer and activator of transcription 3) plays a key role in HDL-induced cardioprotection. In the heart, microRNAs (miRNAs) are involved in ischemia reperfusion injury. We
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实验方案

Trypsin Cell Dissociation Protocol

Trypsin is commonly used for dissociating adherent cells from surfaces. A wide variety of trypsin solutions are available to meet your specific cell line requirements.

StableCell™ Trypsin Solutions

StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.

用胰蛋白酶从培养表面去除贴壁细胞

胰蛋白酶可用于从培养表面去除贴壁细胞。从培养基底物除去细胞的最常用方法是用胰蛋白酶或胰蛋白酶/EDTA溶液处理。

StableCell™胰蛋白酶溶液

StableCell™胰蛋白酶溶液设计用于执行标准胰蛋白酶溶液所实现的细胞解离，但不同的是前者无需等分、冷冻和解冻。这样可以节省大量时间。

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