H9408
β-羟丁酸脱氢酶 来源于勒氏假单胞菌
lyophilized powder, ≥200 units/mg protein
别名:
(R)-3-羟基丁酸:NAD+ 氧化还原酶, 3-HBDH
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥200 units/mg protein
Biological source:
bacterial (Pseudomonas lemoignei)
产品名称
β-羟丁酸脱氢酶 来源于勒氏假单胞菌, lyophilized powder, ≥200 units/mg protein
biological source
bacterial (Pseudomonas lemoignei)
form
lyophilized powder
specific activity
≥200 units/mg protein
purified by
chromatography
foreign activity
lactic dehydrogenase ≤0.05%
malic dehydrogenase ≤0.1%
storage temp.
−20°C
Quality Level
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Application
β-来源于勒氏假单胞菌 的羟丁酸脱氢酶已被用于偶联检测以证实乙酰乙酸酯的形成。
适用于通过Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974)中介绍的方法测定乙酰乙酸酯和D(-)-3-羟基丁酸
Biochem/physiol Actions
在脱氮副球菌中，3-羟丁酸脱氢酶在胞内聚羟基丁酸酯和聚羟基戊酸酯的分解中起关键作用。
在哺乳动物系统中，β-羟丁酸脱氢酶位于线粒体内膜上，需要磷脂酰胆碱才能发挥活性。相反，在假单胞菌属中则为可溶性胞质酶，不需要磷脂别构活化剂。利用作为代谢能量源的酮体时，需要这种酶。其首先催化 3-羟基丁酸酯氧化成乙酰乙酸酯，这是酮体转化为柠檬酸的第一步，而后通过三羧酸循环（Krebs 循环）进一步参与代谢。
General description
D-β-羟丁酸脱氢酶 (BDH) 是一种与膜结合的脂质需求酶。
Other Notes
在pH 7.8、温度37°C条件下，一单位每分钟可将1.0 μmol的 D-β-羟基丁酸氧化成乙酰乙酸盐。
Physical form
含有蔗糖、β-NAD和Tris缓冲盐的冻干粉末
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
此项目有
D-beta-HYDROXYBUTYRATE DEHYDROGENASE: A MOLECULAR BIOLOGY APPROACH TO THE STUDY OF A LIPID-REQUIRING ENZYME
Structure and Function of Membrane Proteins, 283-300 (1983)
3-Hydroxybutyrate oligomer hydrolase and 3-hydroxybutyrate dehydrogenase participate in intracellular polyhydroxybutyrate and polyhydroxyvalerate degradation in Paracoccus denitrificans
Lu J, et al.
Applied and Environmental Microbiology, 80(3), 986-993 (2014)
W Hugo Ramos-Vera et al.
Journal of bacteriology, 193(5), 1201-1211 (2010-12-21)
Two autotrophic carbon fixation cycles have been identified in Crenarchaeota. The dicarboxylate/4-hydroxybutyrate cycle functions in anaerobic or microaerobic autotrophic members of the Thermoproteales and Desulfurococcales. The 3-hydroxypropionate/4-hydroxybutyrate cycle occurs in aerobic autotrophic Sulfolobales; a similar cycle may operate in autotrophic
The specific molecular architecture of plant 3-hydroxy-3-methylglutaryl-CoA lyase
Hemmerlin A, et al.
The Journal of biological chemistry, 80(3) (2019)
J Nie et al.
Scandinavian journal of medicine & science in sports, 21(5), 625-629 (2010-05-13)
This study examined the response of serum biomarkers of cardiac and skeletal muscle damage at rest and after a routine workout of 21 km run in 12 male adolescent (16.2±0.6 years) long-distance runners. Biomarkers of cardiac [troponins (cTnT, cTnI), creatine
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