Merck
CN

H9914

Millipore

HIS-Select® 镍磁性琼脂糖珠

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别名:
nickel charged magnetic beaded agarose
NACRES:
NA.56

偶联物

magnetic beads

质量水平

形式

suspension

保质期

2 yr (Unopened product)

基质

6% beaded magnetic agarose

容量

≥15 mg/mL binding capacity

储存温度

2-8°C

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此商品
P661116-661SAE0197
HIS-Select® Nickel Affinity Gel (1:1 suspension in a 20% ethanol solution)

Millipore

P6611

HIS-Select® 镍亲和凝胶

Magna ChIP® Protein A Magnetic Beads Recombinant Protein A covalently bound to magnetic beads for use in chromatin immunoprecipitations (ChIP assays). These protein A beads provide users a more rapid, reproducible & efficient reagent for collecting immunocomplexes vs. agarose beads.

Sigma-Aldrich

16-661

Magna ChIP® 蛋白A磁珠

Anti-HA Magnetic Beads Magnetic agarose, suspension

Sigma-Aldrich

SAE0197

抗-HA磁珠

form

suspension

form

(1:1 suspension in a 20% ethanol solution)

form

-

form

suspension

shelf life

2 yr (Unopened product)

shelf life

-

shelf life

-

shelf life

-

matrix

6% beaded magnetic agarose

matrix

6% Beaded Agarose

matrix

-

matrix

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

capacity

≥15 mg/mL binding capacity

capacity

>15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

capacity

-

capacity

-

一般描述

HIS-选择性磁性琼脂糖珠由顺磁性的固定化金属离子亲和色谱(IMAC)树脂组成,包含专有的四配位体螯合物,与镍结合,通过不带电荷的亲水性连接子共价附于磁性珠状琼脂糖上。磁珠的磁性有助于操作,例如可反复清洗和回收与蛋白结合的磁珠。这使得实验重现性更高,更准确地定量组氨酸标记的目标蛋白。

应用

HIS-选择性镍磁性琼脂糖珠设计用于组氨酸标记蛋白的自动化和小规模亲和捕获(分子pull-down)纯化,同时表现出与其他蛋白非特异性结合低。该珠可在天然和变性条件下纯化His标签蛋白。

外形

以30%乙醇溶液中的50%浆液悬浮液的形式提供。

法律信息

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

相关产品

产品编号
说明
价格

警示用语:

Danger

危险分类

Aquatic Chronic 3 - Carc. 1B - Flam. Liq. 3 - Repr. 1B - Skin Sens. 1 - STOT RE 2

靶器官

Respiratory Tract

储存分类代码

3 - Flammable liquids

WGK

WGK 3

闪点(°F)

86.0 °F

闪点(°C)

30 °C

法规信息

危险化学品

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Sigma-Aldrich

Sigma-Aldrich

I1408

IMAC-Select Affinity Gel

Anti-FLAG® M2 Magnetic Beads affinity isolated antibody

Millipore

M8823

抗-FLAG® M2磁珠

Philip Vitorino et al.
Nature, 519(7544), 425-430 (2015-03-25)
Cell migration is a stepwise process that coordinates multiple molecular machineries. Using in vitro angiogenesis screens with short interfering RNA and chemical inhibitors, we define here a MAP4K4-moesin-talin-β1-integrin molecular pathway that promotes efficient plasma membrane retraction during endothelial cell migration.
Mei Suen Kong et al.
Science signaling, 12(567) (2019-02-07)
T cell activation is initiated by signaling molecules downstream of the T cell receptor (TCR) that are organized by adaptor proteins. CIN85 (Cbl-interacting protein of 85 kDa) is one such adaptor protein. Here, we showed that CIN85 limited T cell
Sonja Hänzelmann et al.
Clinical epigenetics, 7, 19-19 (2015-03-13)
Primary cells enter replicative senescence after a limited number of cell divisions. This process needs to be considered in cell culture experiments, and it is particularly important for regenerative medicine. Replicative senescence is associated with reproducible changes in DNA methylation
Joshua W Burgess et al.
American journal of respiratory cell and molecular biology, 41(6), 714-721 (2009-03-17)
Pneumocystis organisms are opportunistic fungal pathogens that cause significant pneumonia in immune-compromised hosts. Recent evidence has suggested that Pneumocystis carinii exists as separate mating types, and expresses and regulates proteins that govern meiosis and progression of the life cycle. This
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Journal of materials chemistry. B, 1(15), 2066-2071 (2013-04-21)
The imidazolium cation was chosen as protein selective affinity group and a kind of ionic liquid magnetic microspheres was developed by immobilizing imidazolium cations onto the surface of silica-coated magnetic microspheres to form imidazolium ionic liquid modified magnetic microspheres (Fe3O4@SiO2@IL).

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