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HLP400

Normal Human Hepatic Kupffer Cells

Single-Donor Cryopreserved Kupffer cells isolated from human livers, high viability, and characterized by cell specific markers and response to LPS stimulation

别名:

Homogenous population isolated from whole human livers

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NACRES:
NA.81
UNSPSC Code:
41106509

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产品名称

Normal Human Hepatic Kupffer Cells, Single-Donor Cryopreserved Kupffer cells isolated from human livers, high viability, and characterized by cell specific markers and response to LPS stimulation

biological source

human liver

form

frozen liquid

packaging

pkg of 1 vial (contains ≥0.25 million cells)
pkg of 1 vial (contains ≥0.5 million cells)
pkg of 1 vial (contains ≥1 million cells)

technique(s)

cell culture | mammalian: suitable

color

cloudy

storage temp.

-140 to -196°C

Disclaimer

Each donor is tested and found non-reactive to viral DNA from HIV-1, HIV-2, HTLV I, HTLV II, hepatitis B and hepatitis C. However, no known test can offer complete assurance that the viruses that cause HIV-1, HIV-2, HTLV I, HTLV II, hepatitis B and hepatitis C are not present. Proper precautions and biological containment should be taken when handling cells of human origin, due to their potential biohazardous nature. All human based products should be handled at a BSL-2 (Biosafety Level 2) or higher.
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Features and Benefits

Our cryopreserved hepatic Kupffer cells are derived from the human liver, with each donor providing documented consent for research use of non-transplantable organs or tissues. Following the primary culture, these cells are cryopreserved. Each lot undergoes testing for specific cell markers, fold activation of cytokine production following LPS stimulation and comes with a guarantee of ≥70% post-thaw viability. In addition, all cells have been tested for the absence of HIV, HBV, HCV, Syphillis and microbial contaminants (fungi, bacteria, and mycoplasma).

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Hepatic Kupffer Cells, also known as Browicz-Kupffer cells and stellate macrophages, are specialized macrophages in the liver that line the walls of the sinusoids. Kupffer cells can activate macrophages and are constantly exposed to gut-derived bacteria, microbial debris, and bacterial endotoxins. Hepatic Kupffer cells are intimately involved in the liver′s response to infection, toxins, ischemia, resection, and other stresses. Upon activation, Kupffer cells release various products, including cytokines, prostanoides, nitric oxide, and reactive oxygen species. These released molecules regulate other Kupffer cells and neighboring cells, such as Hepatocytes, Stellate cells, Endothelial cells, and other immune cells that traffic through the liver. Human hepatic Kupffer cells are often characterized using flow cytometry for population distributions, and are positive for CD11b, CD14, and CD68. In vitro, these cells do not proliferate and cannot be passaged.

Application

Kupffer cells are ideal in studies of: chronic liver injury, toxicity, inflammatory response activation, cell-cell signaling, NAFLD and NASH initiation and progression and more. We offer matched sets of Kupffer cells from same donor as Hepatocytes, which can be used for co-culture systems to better simulate the complex microenvironment of the liver and gain insights into various liver-related diseases and conditions.

存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

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商品

Frequently Asked Questions (FAQs) about Hepatocytes and Other Liver Cells

Explore answers to frequently asked questions (FAQs) about culturing primary human hepatocytes and other liver cells including Stellate cells, Kupffer cells, Sinusoidal Endothelial cells, and more.

实验方案

Primary Human Hepatic Kupffer Cell Culture Protocol

Discover our collection of primary human hepatic Kupffer cells and protocol for thawing, plating, and growing Kupffer cells. Find the formulation for Kupffer culture maintenance media.

Primary Human Hepatic Sinusoidal Endothelial Cells Culture Protocol

Discover our collection of primary human hepatic sinusoidal endothelial cells, a protocol for thawing, plating, and growing HHSECs, and a recipe for our HHSEC maintenance media formulation.

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Pre-tested, Cryopreserved, and Ready-to-Screen Human Primary Liver Cells

Explore our primary liver cells collection, including primary human Hepatocytes (PHH), spheroid-qualified Hepatocytes, Kupffer cells, Stellate cells, Cholangiocytes, and Sinusoidal Endothelial cells.

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我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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