The gene importin-8 (IPO8) is mapped to human chromosome 12p11.21. It is a RanGTP-binding protein and associates with nuclear protein complex at the nuclear envelope.

Importin-8 recombinant protein epitope signature tag (PrEST)

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Importin-8 (IPO8) is mainly involved in nuclear import of proteins. Knockdown of IPO8 reduces nuclear import of p65 following tumor necrosis factor-α stimulation, resulting in lower NF-κB activity. IPO8 is required for transforming growth factor -β-induced nuclear import of SMAD4 (mothers against decapentaplegic homolog-4). The signal recognition particle (SRP) assembles in the nucleus and is required for targeting of proteins to the rough endoplasmic reticulum. IPO8 is involved in nuclear import of SRP19, a subunit of mammalian SRP. IPO8 interacts and co-localizes with argonaute (AGO) proteins to cytoplasmic processing bodies in a Ran-dependent manner. IPO8 is important for binding of AGO proteins to mRNA targets. In addition, IPO8 controls nuclear localization of AGO2.

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Corresponding Antigen APREST73963

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany