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I2411

Isocitrate Dehydrogenase 1 (NADP+) human

Name: Isocitrate Dehydrogenase 1 (NADP+) human
Brand: SIGMA

recombinant, expressed in Sf9 cells, ≥90% (SDS-PAGE)

别名:

IDH1, Isocitrate Dehydrogenase Cytoplasmic

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关于此项目

化学文摘社编号:

9028-48-2

UNSPSC Code:

12352204

NACRES:

NA.54

EC 号:

1.1.1.42 (BRENDA, IUBMB)

Assay:

≥90% (SDS-PAGE)

Recombinant:

expressed in Sf9 cells

Concentration:

≥0.30 mg/mL protein (Bradford)

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属性

recombinant

expressed in Sf9 cells

Quality Level

200

assay

≥90% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

47.6 kDa

concentration

≥0.30 mg/mL protein (Bradford)

NCBI accession no.

NM_005896

relevant disease(s)

cancer (lung)

shipped in

dry ice

storage temp.

−70°C

说明

General description

Human Isocitrate Dehydrogenase (IDH1), also known as oxalosuccinate decarboxylase, full length [amino acids 1-414 (end)] with C-terminal FLAG-tag, expressed in Sf9 cells via a baculovirus expression system.

Application

Human Isocitrate Dehydrogenase (IDH1) is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. It may be useful as a biomarker for diagnosis and prognosis prediction of non-small cell lung cancer .

Biochem/physiol Actions

Human Isocitrate Dehydrogenase (IDH1), also known as oxalosuccinate decarboxylase, is a full length amino acid with a C-terminal FLAG-tag and is expressed in Sf9 cells via a baculovirus expression system. IDH1 expression has been correlated with poor survival of non-small cell lung cancer. IDH1 promotes tumor growth .

Physical form

Solution in 45 mM Tris-HCl, 124 mM NaCl, 2.4 mM KCl, 90 μg/ml FLAG peptide, 3 mM DTT, and 10% glycerol at pH 8.0.

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存储类别

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

常规特殊物品

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实验方案

Chiral LC-MS/MS of D- and L-2-Hydroxyglutaric Acid Biomarkers

We describe here a rapid and sensitive method to separate and measure D-2-OHG and L-2-OHG enantiomers using high-resolution mass spectrometry (HRMS) detection.

Identification of isocitrate dehydrogenase 1 as a potential diagnostic and prognostic biomarker for non-small cell lung cancer by proteomic analysis.

Fengwei Tan et al.

Molecular & cellular proteomics : MCP, 11(2), M111-M111 (2011-11-09)

Lung cancer is the leading cause of cancer-related death in the world. To explore tumor biomarkers for clinical application, two-dimensional fluorescence difference gel electrophoresis and subsequent MALDI-TOF/TOF mass spectrometry were performed to identify proteins differentially expressed in 12 pairs of

IDH1 and IDH2 mutations are frequent genetic alterations in acute myeloid leukemia and confer adverse prognosis in cytogenetically normal acute myeloid leukemia with NPM1 mutation without FLT3 internal tandem duplication.

Peter Paschka et al.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 28(22), 3636-3643 (2010-06-23)

To analyze the frequency and prognostic impact of isocitrate dehydrogenase 1 (IDH1) and isocitrate dehydrogenase 2 (IDH2) mutations in acute myeloid leukemia (AML). We studied 805 adults (age range, 16 to 60 years) with AML enrolled on German-Austrian AML Study

Glioma-derived mutations in IDH: from mechanism to potential therapy.

Yuejun Fu et al.

Biochemical and biophysical research communications, 397(2), 127-130 (2010-06-01)

Heterozygous mutations in either the R132 residue of isocitrate dehydrogenase I (IDH1) or the R172 residue of IDH2 in human gliomas were recently highlighted. Heterozygous mutations in the IDH1 occur in the majority of grade II and grade III gliomas

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全球贸易项目编号

货号	GTIN
I2411-10UG	04061833234716