Interleukin-7 (IL-7) is a stromal-cell derived cytokine that is important in the development of adaptive immunity and maintenance of T cells and B cells. The cognate receptor that binds IL-7 is the IL-7 receptor complex, made up of IL-7 R α (CD127) chain and a γ chain. The expression of IL-7 R is restricted to immature B cells, mature T cells and thymocytes. The binding of IL-7 to its receptor triggers a variety of tightly regulated signaling pathways that are critical for cell survival, proliferation, differentiation and uptake of glucose. The signaling pathways that participate in mediating the effects of IL-7 R are JAK/STAT and PI3K. Additionally, the effect of IL-7 on cell cycle is due to the modulation of proteins p27^kip1 and Cdc25A. Reports implicate IL-7 R in glucose metabolism and cell size as it modulates the effects of GLUT4. Anti-interleukin-7 receptor α/CD127 recognizes mouse IL-7 Rα. This antibody shows less than 1% cross reactivity with rhIL-7 Rα.

purified, NSO cell-derived recombinant mouse interleukin-7 soluble receptor α.

Anti-interleukin-7 receptor α/CD127 antibody may be used for immunoblotting at a working concentration of 0.1-0.2 μg/ml. For flow cytometry, the recommended concentration is 3-10 μg/ml/⁶ cells.

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.