Anti-Interleukin-12 antibody produced in goat

Interleukin-12 (IL-12) is an important cytokine that links innate and adaptive immunity. It plays a role in the regulation of Th1-associated cellular immunity to foreign pathogens, and humoral immunity to antigens that are both T cell-independent and T cell-dependent. IL-12 is primarily produced by innate immune cells, dendritic cells, epithelial cells and macrophages. All forms of IL-12 bind to the IL-12R which includes IL-12RbI and IL-12RbII subunits expressed on T cells and natural cells. IL-12 stimulates the production of IFN-γ and TNF-α, subsequent production of nitric oxide that leads to pathogen destruction. Reports suggest the involvement of JAK/STAT pathway components in mediating these effects. The effects of IL-12 have been implicated in bacterial, fungal and viral infections. Due to its role in inducing matrix metalloproteases, it also has important role in the etiology of chronic B-cell malignancies and lung adenocarcinomas
Anti-Interleukin-12 antibody recognizes mouse IL-12. It shows < 10% cross-reactivity with recombinant human IL-12 and < 5% cross-reactivity with recombinant porcine IL-12.

purified, insect cell line Sf21-derived, recombinant mouse IL-12.

Anti-Interleukin-12 antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. The antibody is also suitable for neutralization reactions. It has been used to neutralize IL-12 in the extracts of murine splenocytes.

The antibody will neutralize the biological activity of recombinant mouse IL-12. Based on ELISA, the antibody shows ~10% cross-reactivity with recombinant human IL-12 and <5% cross-reactivity with recombinant porcine IL-12.

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Purified using protein G.

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