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Merck
CN

I8896

Sigma-Aldrich

IGEPAL® CA-630

Molecular Biology

别名:

辛基苯氧基聚（乙烯氧基）乙醇，支链, 聚氧乙烯辛烷基苯酚醚，分支, 辛基苯基-聚乙烯二醇

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线性分子式:
(C2H4O)nC14H22O
化学文摘社编号:
9002-93-1
UNSPSC代码:
12352104
NACRES:
NA.31

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质量水平

200

等级

Molecular Biology

描述

non-ionic

表单

liquid

技术

IR spectroscopy: suitable

CMC

0.08 mM (20-25°C)

粘度

~300 cP(25 °C)

mp

6 °C

溶解性

water: soluble at 20 °C

密度

1.060 g/cm3

HLB

13

异质活性

DNase and RNase, none detected

SMILES字符串

O(CCO)c1ccc(cc1)C(CC(C)(C)C)(C)C

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI key

JYCQQPHGFMYQCF-UHFFFAOYSA-N

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相关类别

一般描述

IGEPAL® CA-630是一种非离子型、非变性去污剂，适用于膜蛋白复合物的溶解、分离和纯化。它与Nonidet P-40在化学上无法区分。

应用

IGEPAL® CA-630已用于：
  • 在小鼠肌肉匀浆液的蛋白质印迹分析(WB)中，作为匀浆缓冲液的中性去垢剂
  • 在E8.5-E10.5胚胎的lacZ表达中，用于X-gal染色
  • 加入细胞裂解(CL)缓冲液中，制备RT-qPCR即用型细胞裂解液

分析说明

在化学性质方面，不能与已经退市的 Nonidet P-40 区分开。

其他说明

非离子型、非变性洗涤剂

法律信息

IGEPAL is a registered trademark of Solvay

免责声明

在贮存过程中可能产生混浊或沉淀物。 可在加热至时40°C变为澄清液体。

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

483.8 °F - closed cup

闪点(°C)

251 °C - closed cup

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCZ1618
MKCX2388
MKCW7585
MKCV4219
MKCR5566

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. I am looking for Nonidet 40, but I am unable to find it. Do you sell this product? If not, can you suggest a suitable alternate product?

    Nonidet 40 or NP40 is no longer commercially available. The Igepal CA-630 (Product No. I8896), is the best substitute product that we can recommend.

  7. Are the 2 products, Igepal CA-630 (Product No. I8896) and Nonidet 40 the same? How do you use this product?

    Igepal CA-630 is chemically indistinguishable to NP40. This non-denaturing detergent is used the same way as that of NP40.

  8. In what form does Product No. I8896, IGEPAL® CA-630, arrive?

    It is a 100% concentrated liquid product. It is not diluted. Please check the Specification sheet available on our product detail page.

  9. How do we solubilize Product No. I8896, IGEPAL® CA-630?

    The product I8896 is miscible in all proportions with water.

  10. What is the formula weight of Product No. I8896, IGEPAL® CA-630?

    The formula weight of this product is 603.

  11. What is the CMC (critical micellar concentration) value of Product No. I8896, IGEPAL® CA-630?

    The CMC value of this product is 0.083 mM.

  12. What is the viscosity of Product No. I8896, IGEPAL® CA-630?

    The viscosity is determined for each lot and the information is available on the lot- specific Certificate of Analysis.

  13. What does "Molecular Biology Grade" mean?

    Molecular Biology Grade means that this product must meet the DNase, RNase, and Nickase specification on the Certificate of Analysis for each lot of the product.  

  14. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Bojana Lucic et al.
Nature communications, 10(1), 4059-4059 (2019-09-08)
HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained
Joseph A Roche et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 59(11), 964-975 (2011-11-02)
Mutations in the DYSF gene that severely reduce the levels of the protein dysferlin are implicated in muscle-wasting syndromes known as dysferlinopathies. Although studies of its function in skeletal muscle have focused on its potential role in repairing the plasma
Amina Abbadi et al.
The Journal of biological chemistry, 291(3), 1448-1455 (2015-11-26)
Many cells, including murine airway epithelial cells, respond to a variety of inflammatory stimuli by synthesizing leukocyte-adhesive hyaluronan (HA) cables that remain attached to their cell surfaces. This study shows that air-liquid interface cultures of murine airway epithelial cells (AECs)
Simon Lalonde et al.
Genome biology, 20(1), 133-133 (2019-07-10)
Genome-wide association studies (GWAS) have identified hundreds of loci associated with coronary artery disease (CAD) and blood pressure (BP) or hypertension. Many of these loci are not linked to traditional risk factors, nor do they include obvious candidate genes, complicating
Per Wasteson et al.
Development (Cambridge, England), 135(10), 1823-1832 (2008-04-18)
Aortic smooth muscle cells (SMCs) have been proposed to derive from lateral plate mesoderm. It has further been suggested that induction of SMC differentiation is confined to the ventral side of the aorta, and that SMCs later migrate to the
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