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I8896

IGEPAL® CA-630

Molecular Biology

别名:

辛基苯氧基聚（乙烯氧基）乙醇，支链, 聚氧乙烯辛烷基苯酚醚，分支, 辛基苯基-聚乙烯二醇

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关于此项目

线性分子式:
(C2H4O)nC14H22O
化学文摘社编号:
9002-93-1
UNSPSC Code:
12352104
NACRES:
NA.31
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Quality Level

200

grade

Molecular Biology

description

non-ionic

form

liquid

technique(s)

IR spectroscopy: suitable

CMC

0.08 mM (20-25°C)

viscosity

~250-350 cP(25 °C)

mp

6 °C

solubility

water: soluble at 20 °C

density

1.060 g/cm3

HLB

13

foreign activity

DNase and RNase, none detected

SMILES string

O(CCO)c1ccc(cc1)C(CC(C)(C)C)(C)C

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI key

JYCQQPHGFMYQCF-UHFFFAOYSA-N

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相关类别

General description

IGEPAL® CA-630是一种非离子型、非变性去污剂，适用于膜蛋白复合物的溶解、分离和纯化。它与Nonidet P-40在化学上无法区分。

Application

IGEPAL® CA-630已用于：
  • 在小鼠肌肉匀浆液的蛋白质印迹分析(WB)中，作为匀浆缓冲液的中性去垢剂
  • 在E8.5-E10.5胚胎的lacZ表达中，用于X-gal染色
  • 加入细胞裂解(CL)缓冲液中，制备RT-qPCR即用型细胞裂解液

Analysis Note

在化学性质方面，不能与已经退市的 Nonidet P-40 区分开。

Other Notes

非离子型、非变性洗涤剂

Legal Information

IGEPAL is a registered trademark of Solvay

Disclaimer

在贮存过程中可能产生混浊或沉淀物。 可在加热至时40°C变为澄清液体。

signalword

Danger

存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

483.8 °F - closed cup

flash_point_c

251 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Irrit. 2

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
MKCZ9158
MKCZ6517
MKCZ1618
MKCX2388
MKCW7585

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Joseph A Roche et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 59(11), 964-975 (2011-11-02)
Mutations in the DYSF gene that severely reduce the levels of the protein dysferlin are implicated in muscle-wasting syndromes known as dysferlinopathies. Although studies of its function in skeletal muscle have focused on its potential role in repairing the plasma
Bojana Lucic et al.
Nature communications, 10(1), 4059-4059 (2019-09-08)
HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained
Amina Abbadi et al.
The Journal of biological chemistry, 291(3), 1448-1455 (2015-11-26)
Many cells, including murine airway epithelial cells, respond to a variety of inflammatory stimuli by synthesizing leukocyte-adhesive hyaluronan (HA) cables that remain attached to their cell surfaces. This study shows that air-liquid interface cultures of murine airway epithelial cells (AECs)
Kenneth Shatzkes et al.
Scientific reports, 4, 4659-4659 (2014-04-12)
Sample nucleic acid purification can often be rate-limiting for conventional quantitative PCR (qPCR) workflows. We recently developed high-throughput virus microneutralization assays using an endpoint assessment approach based on reverse transcription qPCR (RT-qPCR). The need for cumbersome RNA purification is circumvented
Per Wasteson et al.
Development (Cambridge, England), 135(10), 1823-1832 (2008-04-18)
Aortic smooth muscle cells (SMCs) have been proposed to derive from lateral plate mesoderm. It has further been suggested that induction of SMC differentiation is confined to the ventral side of the aorta, and that SMCs later migrate to the
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实验方案

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

RIP试剂盒的哺乳动物细胞Ago抗体-RNA免疫沉淀实验方案

使用RIP试剂盒进行哺乳动物细胞Ago抗体-RNA免疫沉淀的实验方案和流程

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蛋白质Pull-Down技术

利用亲和力、GST pull-down、TAP 和共免疫沉淀方法，通过Pull-Down研究体外蛋白质互作。

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

全球贸易项目编号

货号GTIN
I8896-50ML04061838256003
I8896-100ML04061838255990

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