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Merck
CN

I9516

Sigma-Aldrich

2-丙醇

≥99.5%, for molecular biology, suitable for RNA extraction, DNA extraction, BioReagent

别名:

IPA, 仲丙醇, 异丙醇

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关于此项目

线性分子式:
(CH3)2CHOH
CAS Number:
67-63-0
分子量:
60.10
Beilstein:
635639
EC 号:
200-661-7
MDL编号:
MFCD00011674
UNSPSC代码:
12352104
PubChem化学物质编号:
24896158
NACRES:
NA.52

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Product Name

2-丙醇, BioReagent, ≥99.5%, Molecular Biology

等级

Molecular Biology

质量水平

200

蒸汽密度

2.1 (vs air)

蒸汽压

33 mmHg ( 20 °C)
44 mmHg ( 25 °C)

产品线

BioReagent

方案

≥99.5%

表单

liquid

自燃温度

750 °F

expl. lim.

2.0-12.7 %, 93 °C

技术

DNA extraction: suitable
RNA extraction: suitable

杂质

≤0.2% water
water

颜色

colorless

折射率

n20/D 1.377 (lit.)

沸点

82 °C (lit.)

mp

−89.5 °C (lit.)

溶解性

water: soluble (completely)

密度

0.785 g/mL at 25 °C (lit.)

适用性

suitable for precipitation of nucleic acids

储存温度

room temp

SMILES字符串

CC(C)O

InChI

1S/C3H8O/c1-3(2)4/h3-4H,1-2H3

InChI key

KFZMGEQAYNKOFK-UHFFFAOYSA-N

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相关类别

一般描述

异丙醇是一种透明、无色、极性的有机溶剂，常用于化学和分子生物学实验室。它可溶解一系列的化物品并快速蒸发。本品（2-丙醇）为分子生物学级，适用于核酸沉淀。

异丙醇使用方案：

  • 从哺乳动物组织沉淀DNA，从DNA样品中提取溴化乙锭，以及从细胞和组织中分离RNA。
  • 蛋白质结晶。
  • 从生物基质中分离类维生素A，进行HPLC和HPLC-MS分析。

应用

2-丙醇已用于：
  • DNA和RNA萃取
  • 测定羟脯氨酸水平（《The Merck Index》第12版）
  • MTT测定检验细胞活力
  • 芯片杂交
适用于使用标准实验流程用于DNA沉淀。还适合作为针对分子生物学应用所制备溶液中的一种溶剂。

象形图

FlameExclamation mark
GHS02,GHS07

警示用语：

Danger

危险声明

H225,H319,H336

危险分类

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

靶器官

Central nervous system

储存分类代码

3 - Flammable liquids

WGK

WGK 1

闪点(°F)

53.6 °F - closed cup

闪点(°C)

12.0 °C - closed cup

个人防护装备

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

危险化学品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000482871
0000482871
SHBT0929
0000473250
0000473250

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F Yang et al.
Journal of molecular biology, 288(3), 403-412 (1999-05-18)
The crystal structure of cyanovirin-N (CV-N), a protein with potent antiviral activity, was solved at 1.5 A resolution by molecular replacement using as the search model the solution structure previously determined by NMR. The crystals belong to the space group
S Knapp et al.
Proteins, 23(4), 595-597 (1995-12-01)
The thermostable amylopullulanase from Pyrococcus woesei was crystallized. Crystals, suitable for a crystallographic analysis up to a size of 0.6 mm in their longest dimension, have been obtained by the vapor diffusion method in a solution containing polyethyleneglycol 4000 (PEG
Robert B Nicewonger et al.
Bioorganic & medicinal chemistry letters, 12(14), 1799-1802 (2002-06-28)
In this paper, we describe the synthesis of N-(6-cyano-1,3-dimethyl-2,4-dioxo-5-substituted-1,3-dihydropyridino[2,3-d] pyrimidin-7-yl)imides 1. We will show the synthesis of 1 using both conventional heating and microwave techniques. In addition, the imide was attached to polystyrene and this immobilized imide was equally as
M J Romão et al.
European journal of biochemistry, 215(3), 729-732 (1993-08-01)
The Desulfovibrio gigas aldehyde oxidoreductase contains molybdenum bound to a pterin cofactor and [2Fe-2S] centers. The enzyme was characterized by SDS/PAGE, gel-filtration and analytical ultracentrifugation experiments. It was crystallized at 4 degrees C, pH 7.2, using isopropanol and MgCl2 as
Christudas Morais et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 25(5), 1462-1474 (2009-12-29)
Renal cell carcinoma (RCC) is a highly metastatic and lethal disease with few efficacious treatments. Many studies have shown that the ubiquitous transcription factor nuclear factor kappa B (NF-kappaB) plays a key role in the development and progression of many
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