产品名称
IgG 来源于牛血清, technical grade, ≥80% (SDS-PAGE), buffered aqueous solution
conjugate
unconjugated
grade
technical grade
assay
≥80% (SDS-PAGE)
form
buffered aqueous solution
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Application
IgG from bovine serum is used as a supplement in cell culture.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Bovine immunoglobulin, protein source obtained from immunoglobulin derived from serum, is a naturally occurring bioactive proteins and peptides. Fetal calf or bovine serum IgG can be depleted with protein G affinity chromatography and various concentration of this product can be used in RPMI-1640 medium to produce mouse hybridoma cell line CAy-G (anti-HBs IgG1 mAb producing hybridoma cell). The heat aggregated bovine serum IgG is also useful in down regulating mannose receptor expression. IgG from bovine serum reacts specifically with mouse.
Immunogen
IgG is isolated from pooled bovine serum.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.2 with 15 mM sodium azide as preservative
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
含少量动物源组分生物产品
此项目有
S Schreiber et al.
Journal of immunology (Baltimore, Md. : 1950), 147(4), 1377-1382 (1991-08-15)
We previously described the presence of an inhibitory protein contained in the 20 to 40% (NH4)2SO4 precipitable fraction of FCS that down-regulates expression of mannose receptors on bone marrow-derived macrophages. We now identify aggregated bovine IgG as the main inhibitory
C Aybay et al.
Journal of immunological methods, 233(1-2), 77-81 (2000-01-29)
Fetal calf serum (FCS) was depleted of its immunoglobulin G (IgG) in a rapid procedure using protein G affinity chromatography. 20 ml of FCS was depleted of its IgG in less than 80 min by applying 5 ml of FCS
Manita Shakya et al.
Endocrinology (2021-03-12)
Pro-opiomelanocortin (POMC) neurons form an integral part of the central melanocortin system regulating food intake and energy expenditure. Genetic and pharmacological studies have revealed that defects in POMC synthesis, processing, and receptor signaling lead to obesity. It is well established
Carlos Mendoza-Palomares et al.
Eukaryotic cell, 7(4), 684-697 (2008-02-19)
Cysteine proteases have been shown to be essential virulence factors and drug targets in trypanosomatids and an attractive antidisease vaccine candidate for Trypanosoma congolense. Here, we describe an important amplification of genes encoding cathepsin B-like proteases unique to T. congolense.
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