Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
pH
6.1-6.7
solubility
water: 9.5 g/L, clear
storage temp.
2-8°C
General description
Krebs-Ringer碳酸氢盐缓冲液作为盐溶液在组织培养中起重要作用。盐溶液用于供给细胞水和无机离子。还可维持培养基的渗透平衡和理想的pH水平。这种碳酸氢盐缓冲等渗盐水含有镁、钾、钠和磷酸根离子。
Application
Krebs-Ringer碳酸氢盐缓冲液已用于：
- 作为洗涤缓冲液，用于原代脂肪细胞的制备和脂解分析
- 作为预灌流溶液组分，用于胰岛活力和功能研究
- 垂体的生物学分析
Preparation Note
并补充1.26g/L碳酸氢钠。
配方中每升培养基含有9.5克的粉末。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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实验方案
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
粉末培养基及盐混合物极具吸湿性，应注意防潮。
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