Terminal deoxynucleotidyl Transferase (TdT)

Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3′ hydroxyl terminus of single or double stranded DNA molecules. The presence of 1 mM Co²⁺ stimulates the tailing of the 3′-ends of DNA fragments. This construct is sold as an N-terminal truncation of the terminal transferase gene attached to an N-terminal fusion tag.

Suitable for:

• Homopolymeric tailing to the 3′ OH
• TUNEL assay
• 5′-RACE
• Labeling of 3′ ends

Supplied in 50 mM KPO₄, 100 mM NaCl, 1.0 mM DTT, 0.1 mM EDTA, 0.1% Triton^™ X-100, and 50% glycerol at pH 7.3 @ 25° C.

1 unit is defined as the amount of polymerase required to convert 1 nmol of dTTPs into acid insoluble material in 1 hour at 37° C.

Source of protein: An E. coli strain that carries the cloned terminal transferase gene from calf thymus.

Supplied with:KEM0043B (10X Green Buffer)KEM0045B (2.5 mM CoCl₂

Unit size: 6,000 U

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