L0159
抗荧光素酶 兔抗
IgG fraction of antiserum, buffered aqueous solution
别名:
Anti Luciferase Antibody, Firefly Luciferase Antibody, Luciferase Antibody, Luciferase Antibody - Anti-Luciferase antibody produced in rabbit
产品名称
抗荧光素酶 兔抗, IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
packaging
antibody small pack of 25 μL
technique(s)
indirect immunofluorescence: 1:500-1:1,000 using eukaryotic cells transfected with a plasmid bearing the luciferase gene
western blot: 1:10,000 using purified Luciferase
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Application
兔抗萤光素酶抗体可用于免疫印迹和免疫组化。
Biochem/physiol Actions
该抗体识别携带荧光素酶基因的质粒转染的真核细胞中的重组荧光素酶。
Disclaimer
除非我们的目录或产品随附的其他公司文档中另有说明，我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人或动物的任何类型的消费或应用。
General description
基因表达分析通常通过瞬时转染进行分析。这些系统通常基于使用插入细胞的融合基因，并在引入 DNA 后 48 小时内分析基因的表达。通常，融合由研究中的启动子结合位点或增强子序列组成，其与报告基因连接。在实验条件下合成的报告蛋白的量推测反映了所研究序列直接或促进转录的能力。
荧光素酶已成为应用较为广泛的报告酶之一。报告质粒含有来自萤火虫的基因。该酶催化生物发光反应，该反应需要底物荧光素以及Mg +2 +和ATP。将这些试剂与含有荧光素酶的细胞提取物混合，会导致闪光快速衰减。这种光可用光度计检测到。总光发量与样品的荧光素酶活性成正比。荧光素酶试验快速且灵敏，不需要像 CAT 试验那样的放射性底物。荧光素酶测定的缺点是它需要相当昂贵的仪器，即发光计来测量酶活性。
荧光素酶已成为应用较为广泛的报告酶之一。报告质粒含有来自萤火虫的基因。该酶催化生物发光反应，该反应需要底物荧光素以及Mg +2 +和ATP。将这些试剂与含有荧光素酶的细胞提取物混合，会导致闪光快速衰减。这种光可用光度计检测到。总光发量与样品的荧光素酶活性成正比。荧光素酶试验快速且灵敏，不需要像 CAT 试验那样的放射性底物。荧光素酶测定的缺点是它需要相当昂贵的仪器，即发光计来测量酶活性。
Immunogen
萤火虫 (Photinus pyralis) 荧光素酶。
Physical form
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
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