description
with Toluene
Quality Segment
type
for DNA synthesis
storage condition
dry at room temperature
concentration
42.2-51.6 g/L (DCA content, titration)
impurities
≤200 ppm water content (Karl Fischer)
color
clear
compatibility
configured for (ÄKTA® and OligoPilot®)
General description
Deblock solution containing dichloroacetic acid (DCA) in toluene removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. This solution contains toluene / Dichloroactic Acid (97/3 v/v).
Application
DCA Deblock solution facilitates removal of the DMT that allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.
Preparation Note
甲苯/二氯乙酸 97/3 (v/v)
Legal Information
OligoPilot is a registered trademark of Cytiva
ÄKTA is a registered trademark of Cytiva
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signalword
Danger
Hazard Classifications
Aquatic Chronic 3 - Asp. Tox. 1 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Lact. - Repr. 1B - Skin Irrit. 2 - STOT RE 2 Inhalation - STOT SE 3
target_organs
Central nervous system
存储类别
3 - Flammable liquids
flash_point_f
39.2 °F - closed cup
flash_point_c
4 °C - closed cup