conjugate
FITC conjugate
form
lyophilized powder
potency
<20 μg per mL agglutination activity
composition
Protein, ≥95% biuret
extent of labeling
2-4 mol FITC per mol protein (Lowry).
impurities
salt, free
storage temp.
−20°C
Quality Level
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General description
豌豆(Pea)凝集素是一种约48kda的四聚糖蛋白。它是从豌豆中分离得到。豌豆凝集素对α-D-葡萄糖和α-D-甘露糖具有特异性。
Application
豌豆(Pea)凝集素用于确定顶体状态和精子活力以及评估精子顶体反应(AR)模式
Biochem/physiol Actions
PSA不是血型特异性的,但对末端α-D-甘露糖残基具有亲和力。 PSA是一种类似于伴刀豆球蛋白A的促分裂原。
来自豌豆(Pea)的凝集素对淋巴细胞(如刀豆蛋白A (Con A))具有抗肝癌作用和丝裂原活性。
Preparation Note
由亲和纯化凝集素制备偶联物。
Analysis Note
凝集活性以μg/ml表示,并且是通过1mg/ml溶液的系列稀释液(pH 6.8)(在磷酸盐缓冲盐水中进行稀释)进行测定。该活性是在25℃孵育1小时后,对2%人红细胞悬浮液进行凝集所需的最低浓度。
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
低风险生物材料
此项目有
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L S Castro et al.
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