conjugate
FITC conjugate
form
lyophilized powder
potency
<20 μg per mL agglutination activity
composition
Protein, ≥95% biuret
extent of labeling
2-4 mol FITC per mol protein (Lowry).
impurities
salt, free
storage temp.
−20°C
Quality Level
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General description
豌豆(Pea)凝集素是一种约48kda的四聚糖蛋白。它是从豌豆中分离得到。豌豆凝集素对α-D-葡萄糖和α-D-甘露糖具有特异性。
Application
豌豆(Pea)凝集素用于确定顶体状态和精子活力以及评估精子顶体反应(AR)模式
Biochem/physiol Actions
PSA不是血型特异性的，但对末端α-D-甘露糖残基具有亲和力。 PSA是一种类似于伴刀豆球蛋白A的促分裂原。
来自豌豆(Pea)的凝集素对淋巴细胞（如刀豆蛋白A (Con A)）具有抗肝癌作用和丝裂原活性。
Preparation Note
由亲和纯化凝集素制备偶联物。
Analysis Note
凝集活性以μg/ml表示，并且是通过1mg/ml溶液的系列稀释液（pH 6.8）（在磷酸盐缓冲盐水中进行稀释）进行测定。该活性是在25℃孵育1小时后，对2％人红细胞悬浮液进行凝集所需的最低浓度。
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
低风险生物材料
此项目有
In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen
Aires VA, et al.
Theriogenology, 60(2), 269-279 (2003)
Amar K Chandra et al.
Environmental toxicology, 32(6), 1823-1835 (2017-02-17)
Excess iodine induced public health problems are now emerging in many iodine sufficient regions for indiscriminate intake of iodine through various iodized products. It has been reported that excess iodine can disrupt overall male reproductive physiology by generating oxidative stress
Lectin-binding analysis of the biofilm exopolymeric matrix carbohydrate composition of corrosion-aggressive bacteria
Purish LM, et al.
Applied Biochemistry and Microbiology, 49(5), 458-463 (2013)
Crypticity and functional distribution of the membrane associated ?-L-fucosidase of human sperm
Venditti JJ, et al.
Molecular Reproduction and Development, 74(6), 758-766 (2007)
Julio A Martinez-Rodriguez et al.
Reproduction & fertility, 1(1), 11-19 (2020-11-04)
Cryopreservation compromises the capacity of sperm fertilizing due to a series of alterations in the structure and physiology of the sperm. The use of antioxidants, such as melatonin, added to freezing media, may help to reduce sperm cryoinjury. To test
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