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Merck
CN

L0770

来自Pisum sativum(豌豆)的凝集素

FITC conjugate, lyophilized powder

别名:

Pisum sativum凝集素, PSA

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关于此项目

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
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conjugate

FITC conjugate

form

lyophilized powder

potency

<20 μg per mL agglutination activity

composition

Protein, ≥95% biuret

extent of labeling

2-4 mol FITC per mol protein (Lowry).

impurities

salt, free

storage temp.

−20°C

Quality Level

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General description

豌豆(Pea)凝集素是一种约48kda的四聚糖蛋白。它是从豌豆中分离得到。豌豆凝集素对α-D-葡萄糖和α-D-甘露糖具有特异性。

Application

豌豆(Pea)凝集素用于确定顶体状态和精子活力以及评估精子顶体反应(AR)模式

Biochem/physiol Actions

PSA不是血型特异性的,但对末端α-D-甘露糖残基具有亲和力。 PSA是一种类似于伴刀豆球蛋白A的促分裂原。
来自豌豆(Pea)的凝集素对淋巴细胞(如刀豆蛋白A (Con A))具有抗肝癌作用和丝裂原活性。

Preparation Note

由亲和纯化凝集素制备偶联物。

Analysis Note

凝集活性以μg/ml表示,并且是通过1mg/ml溶液的系列稀释液(pH 6.8)(在磷酸盐缓冲盐水中进行稀释)进行测定。该活性是在25℃孵育1小时后,对2%人红细胞悬浮液进行凝集所需的最低浓度。

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

低风险生物材料
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历史批次信息供参考:

分析证书(COA)

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Gediendson Ribeiro de Araujo et al.
Animal reproduction science, 163, 56-62 (2015-11-04)
We evaluated the capacity of ocelot and oncilla spermatozoa to bind to the perivitelline membranes (PVMs) of hen eggs in a sperm binding assay (S-PVM). In addition, a device that improves the standardization of the assay was developed. The number
Yi Zhou et al.
Nature communications, 13(1), 2906-2906 (2022-05-26)
There is evidence to suggest that the primary tumor induces the formation of a pre-metastatic niche in distal organs by stimulating the production of pro-metastatic factors. Given the fundamental role of the pre-metastatic niche in the development of metastases, interruption
The biological significance of phospholipase C beta 1 gene mutation in mouse sperm in the acrosome reaction, fertilization, and embryo development.
Choi D, et al.
Journal of Assisted Reproduction and Genetics, 18(5), 305-310 (2001)
Tácia Gomes Bergstein-Galan et al.
Theriogenology, 96, 69-75 (2017-05-24)
This study investigates the effect of time of storage of epididymides at room temperature and the addition of 20% of seminal plasma to the cryopreservation extender, on post thaw quality and fertility of ovine spermatozoa collected from the cauda epididymis.
L S Castro et al.
Journal of animal science and biotechnology, 7, 17-17 (2016-03-08)
In order to improve the efficiency of bovine sperm cryopreservation process, it is important to understand how spermatozoa respond to differences in temperature as well as the ability to recover its own metabolism. The combination between flow cytometry approach and

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