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Merck
CN

L0770

Sigma-Aldrich

来自Pisum sativum(豌豆)的凝集素

FITC conjugate, lyophilized powder

别名:

Pisum sativum凝集素, PSA

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UNSPSC代码:
12352202
NACRES:
NA.32
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偶联物

FITC conjugate

质量水平

表单

lyophilized powder

效能

<20 μg per mL agglutination activity

组成

Protein, ≥95% biuret

标记范围

2-4 mol FITC per mol protein (Lowry).

杂质

salt, free

储存温度

−20°C

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一般描述

豌豆(Pea)凝集素是一种约48kda的四聚糖蛋白。它是从豌豆中分离得到。豌豆凝集素对α-D-葡萄糖和α-D-甘露糖具有特异性。

应用

豌豆(Pea)凝集素用于确定顶体状态和精子活力以及评估精子顶体反应(AR)模式

生化/生理作用

PSA不是血型特异性的,但对末端α-D-甘露糖残基具有亲和力。 PSA是一种类似于伴刀豆球蛋白A的促分裂原。
来自豌豆(Pea)的凝集素对淋巴细胞(如刀豆蛋白A (Con A))具有抗肝癌作用和丝裂原活性。

制备说明

由亲和纯化凝集素制备偶联物。

分析说明

凝集活性以μg/ml表示,并且是通过1mg/ml溶液的系列稀释液(pH 6.8)(在磷酸盐缓冲盐水中进行稀释)进行测定。该活性是在25℃孵育1小时后,对2%人红细胞悬浮液进行凝集所需的最低浓度。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

法规信息

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分析证书(COA)

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In vitro and in vivo comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of bovine semen
Aires VA, et al.
Theriogenology, 60(2), 269-279 (2003)
Martin Blomberg Jensen et al.
Nature communications, 12(1), 2450-2450 (2021-04-25)
Infertile men have few treatment options. Here, we demonstrate that the transmembrane receptor activator of NF-kB ligand (RANKL) signaling system is active in mouse and human testis. RANKL is highly expressed in Sertoli cells and signals through RANK, expressed in
Lectin-binding analysis of the biofilm exopolymeric matrix carbohydrate composition of corrosion-aggressive bacteria
Purish LM, et al.
Applied Biochemistry and Microbiology, 49(5), 458-463 (2013)
Amar K Chandra et al.
Environmental toxicology, 32(6), 1823-1835 (2017-02-17)
Excess iodine induced public health problems are now emerging in many iodine sufficient regions for indiscriminate intake of iodine through various iodized products. It has been reported that excess iodine can disrupt overall male reproductive physiology by generating oxidative stress
Julio A Martinez-Rodriguez et al.
Reproduction & fertility, 1(1), 11-19 (2020-11-04)
Cryopreservation compromises the capacity of sperm fertilizing due to a series of alterations in the structure and physiology of the sperm. The use of antioxidants, such as melatonin, added to freezing media, may help to reduce sperm cryoinjury. To test

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