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Merck
CN

L1668

来自 菜豆的凝集素 （红芸豆）

Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

别名:

PHA, 植物血球凝集素(菜豆)

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NACRES:
NA.32
UNSPSC Code:
12352202

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产品名称

来自 菜豆的凝集素 （红芸豆）, Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

product line

BioReagent

form

lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

potency

≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

technique(s)

cell culture | mammalian: suitable

color

white to off-white

solubility

PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)

storage temp.

2-8°C

Quality Level

200

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Analysis Note

其效力和溶解度以mg凝集素/ml表示

Application

来自菜豆（Phaseolus vulgaris） （红芸豆）的凝集素已被用于：
  • 刺激分化簇（CD4+）T细胞
  • 激活原发性外周血淋巴细胞
  • 刺激外周血单核细胞

Biochem/physiol Actions

PHA由两种分子种类组成，即具有低促有丝分裂活性和高红细胞凝集素活性的红细胞凝集素（PHA-E），和具有高促有丝分裂和白细胞凝集活性但具有非常低的红细胞凝集活性的白细胞凝集素（PHA-L）。
PHA-E不具有血型特异性，但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前，PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。

Packaging

根据凝集素的含量进行出售

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000484893
0000484893
0000408863
0000406766
0000384022

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How can Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder, be dissolved?

    Product No. L1668 is solubility tested using 5 mg per mL of phosphate buffered saline (PBS), pH 6.8; this yields a clear to slightly hazy, colorless to light yellow solution.

  6. What is the mitogenic activity of Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder?

    The minimum specification for mitogenic activity of this product is  < or = 10 micrograms lectin/mL of medium. The reported specification is PASS. A specific value is not given.

  7. What is the solution stability for Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder?

    The solution stability was not evaluated by Sigma-Aldrich; however, references indicate media containing dilute PHA can be stored refrigerated for 2-4 weeks. PHA loses approximately 30% activity after 1 month. Stock solutions can be stored in frozen aliquots.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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