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L2020

Mouse Laminin

from Engelbreth-Holm-Swarm murine sarcoma basement membrane, 0.2 μm filtered, liquid, 1-2 mg/mL, suitable for cell culture

别名:

层粘蛋白

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关于此项目

化学文摘社编号:
114956-81-9
UNSPSC Code:
12352202
NACRES:
NA.75
MDL number:
MFCD00081739
Biological source:
mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)
Form:
aqueous solution
Technique(s):
cell culture | mammalian: suitable
Concentration:
1-2 mg/mL in Tris-buffered saline
Mol wt:
A subunit 400 kDa, B1 subunit 210 kDa, B2 subunit 200 kDa
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产品名称

层粘连蛋白 来源于 Engelbreth-Holm-Swarm 小鼠肉瘤基底膜, 1-2 mg/mL in Tris-buffered saline, 0.2 μm filtered, BioReagent, suitable for cell culture

biological source

mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)

Quality Level

300

product line

BioReagent

form

aqueous solution

mol wt

A subunit 400 kDa, B1 subunit 210 kDa, B2 subunit 200 kDa

packaging

pkg of 1 mg

concentration

1-2 mg/mL in Tris-buffered saline

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑2 μg/cm2

impurities

Microbial Contamination, passes test

NCBI accession no.

NM_008480.2

UniProt accession no.

P02468, P02469, P19137, Q61092

binding specificity

Peptide Source: Collagen

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Lama1(16772), Lamb1(16777), Lamc1(226519), Lamc2(16782)

相关类别

General description

层粘连蛋白是分子量较大的非胶原粘着糖蛋白。它们是异三聚体蛋白，含有三个二硫键连接的多肽：α、β和 γ链。层粘连蛋白的多个多肽结构域可结合多个细胞表面受体，包括多个整联蛋白二聚体、肌营养不良蛋白聚糖和多配体蛋白聚糖，以及其它细胞外基质成分。这种细胞外基质多结构域三聚体糖蛋白是维持粘附、增殖和分化的基底膜的主要组分。

Application

来自Engelbreth-Holm-Swarm小鼠肉瘤基底膜的层粘连蛋白已用于：
  • 胰岛素生成细胞（IPC）分化
  • 感染抑制检测。
  • 增殖检测。
层粘连蛋白支持多种细胞类型的生长和分化，包括表皮、内皮、神经、肌肉和肝脏细胞。 建议将其按1-2 μg/cm2用作细胞培养的基质。 最佳的浓度的确取决于细胞类型以及应用或研究目标。

Biochem/physiol Actions

层粘连蛋白是动物组织中结构性支架的组成部分。 它们通过内功素和基底膜聚糖与IV型胶原结合，并通过整合素受体、dystroglycan糖蛋白复合物以及Lutheran血型糖蛋白与细胞膜结合。 层粘连蛋白具有胶原结合、细胞粘附、肝素结合和神经突向外生长片段的活性结构域。

Preparation Note

本品以1 − 2 mg/mL浓度形式提供，含50 mM Tris-HCl和150 mM NaCl。 其经0.2μm滤器过滤。在使用前在2-8°C将该溶液缓慢解冻以避免形成凝胶。 如需用作涂层，可在平衡盐溶液中稀释并用最小的体积涂覆在培养的表面，随后在37℃下孵育2小时。 在细胞铺板之前用PBS洗涤3次。层粘连蛋白可储存在2-8°C达一个月。

Other Notes

2024年CiteAb奖——成功的帕金森研究供应商(2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research)
层粘连蛋白是一种细胞外基质多结构域三聚体糖蛋白，并且是支持粘附、增殖和分化的基底膜的主要非胶原组分。 层粘连蛋白是由通过多个二硫键连接的A、B1和B2链组成。这款层粘连蛋白产品是从小鼠Engelbreth-Holm-Swarm肿瘤中分离的。

Disclaimer

建议将其按工作溶液进行分装并储存在-20°C。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

低风险生物材料

此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000519327
0000533006
0000533002
0000533004
0000519327

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用于细胞培养的层粘连蛋白包被实验方案

用于细胞培养的层粘连蛋白包被表面需要特定的条件才能获得理想的结果。包含了用于包被盖玻片以进行神经球状体和常规细胞培养的实验方案。

全球贸易项目编号

货号GTIN
L2020-1MG04061838447289

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