form
lyophilized powder
potency
<20 μg per mL agglutination activity (using human blood group B erythrocytes)
composition
Protein, ≥90% biuret
impurities
salt, free
storage temp.
2-8°C
Quality Level
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Biochem/physiol Actions
BS-I对末端α-D-半乳糖残基具有主要亲和力，对末端N-乙酰基-α-D-半乳糖胺残基具有二级亲和力。
Preparation Note
Purified by affinity chromatography using the method of Hayes, C. E., and Goldstein, I. J., J. Biol. Chem., 249, 1904 (1974).
Analysis Note
凝集活性以 μg/mL表示，并且使用磷酸盐缓冲盐水（pH6.8）对1mg/mL溶液进行连续稀释液来测定，对于每种凝集素，所含有钙、镁和锰的浓度不同。该活性是在25℃孵育1小时后，对2％合适的红细胞悬浮液进行凝集所需的最低浓度。
Other Notes
BS-I是一种四聚体凝集素，由两种类型的亚基A和B组成。存在由不同亚基组成的五种BS-I同工凝集素：BSI-B4、BSI-AB3、BSI-A2B2、BSI-A3B、以及BSI-A4。BSI-B4 是血型B特异的，其对末端α-D-半乳糖残基具有独特的亲和力，而BSI-A4 具有血型A特异性，其对末端N-乙酰基-α-D-半乳糖胺残基具有主要亲和力。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
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