溶壁酶 来源于藤黄节杆菌

藤黄节杆菌（Arthrobacter luteus ）裂解酶已用于裂解真菌细胞壁，分离DNA。

Lyticase from Arthrobacter luteus has been used:

• for spheroplasting the cells
• as a component of digestion solution to incubate yeast cells for digestion of the cell wall
• in the enzymatic hydrolysis of the mycelium precipitate to prepare protoplasts

酵母细胞很难被破坏，因为其细胞壁可能形成胶囊或耐药孢子。可通过裂解酶（如溶壁酶）诱导部分原生质球形成，从酵母中提取DNA。随后再裂解原生质球以释放DNA。溶壁酶更易消化酵母菌细胞壁，并从真菌中产生原生质球进行转化。它含有β-(1→3)-葡聚糖昆布五糖水解酶以及β-(1→3)-葡聚糖酶、蛋白酶和甘露聚糖酶活性。溶壁酶适用于多种酵母细胞，例如念珠菌(Candida)、德巴利酵母菌( Debaryomyces)、酿酒酵母(Saccharomyces)、覆膜酵母(Saccharomycopsis)、类酵母酵(Saccharomycodes)、出鞘菌属(Eremothecium)和许旺酵母(Schwanniomyces species)。

Lyticase enzyme is frequently used in fungal research, particularly for species identification using polymerase chain reaction (PCR)-based techniques. It can break down β (1→3) and β (1→4) bonds between glucose units. Lysozyme serves as an indicator of macrophage-mediated host response, correlates with white cell death, and exhibits a high turnover rate. Elevated levels of serum lysozyme have been observed in various chronic inflammatory conditions, inflammatory bowel diseases, hematological disorders, and renal disorders. The c-type lysozyme from hen egg white is commonly used as a model for studying protein structure and function. Muramidase primarily exhibits bactericidal activity against Gram-positive bacteria. The method described in the bulletin for determining molecular masses using gel filtration chromatography is a modified version of existing published techniques. The protein standards included in this kit may be compatible with other chromatographic systems like high-performance liquid chromatography (HPLC). However, certain buffer systems may affect the elution volumes of albumin and carbonic anhydrase. The proteins in this kit have a molecular mass range spanning from 29 kDa to 699 kDa.

溶细胞酶水解聚-β(1→3)-葡萄糖，如酵母细胞壁葡聚糖。

一个单位在25°C和pH 7.5条件下，在3ml反应混合物中以酵母悬浮液为底物，每分钟产生0.001的ΔA₈₀₀ 。

部分纯化的冻干粉末，含有磷酸钾缓冲盐和稳定剂

更多关于复杂碳水化合物分析酶的信息请见www.sigma-aldrich.com/enzymeexplorer

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.