产品名称
来自西非单叶豆（加纳籽）的凝集素, Isolectin B4 (BSI-B4), FITC conjugate, lyophilized powder
conjugate
FITC conjugate
form
lyophilized powder
potency
<50 μg per mL agglutination activity (using human blood group B erythrocytes)
composition
Protein, ≥70% Lowry (, balance sodium citrate)
extent of labeling
≥1 mol FITC per mol lectin
storage temp.
2-8°C
Quality Level
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Analysis Note
凝集活性以 μg/mL表示，并且使用磷酸盐缓冲盐水（pH6.8）对1mg/mL溶液进行连续稀释液来测定，对于每种凝集素，所含有钙、镁和锰的浓度不同。该活性是在25℃孵育1小时后，对2％合适的红细胞悬浮液进行凝集所需的最低浓度。
Application
来自西非单叶豆（加纳籽）的凝集素已用于鼠星形胶质细胞培养物中的小胶质细胞检测，检测方法是采用凝集BS1-B4对培养物进行染色。它还用于制备孵育组织切片的染色溶液。
Biochem/physiol Actions
BS-I对末端α-D-半乳糖残基具有主要亲和力，对末端N-乙酰基-α-D-半乳糖胺残基具有二级亲和力。
目前确知凝集素可用于鉴定、成像和靶向糖缀合物。由于与寡糖、糖蛋白和糖脂存在特异性相互作用，其还可用于芯片分析，实现有效的糖组图谱分析。在植物和真菌体内，凝集素可防御病原体/寄生物。凝集素参与宿主识别和组织粘附，有助于探索微生物的病理。
General description
凝集素是碳水化合物结合蛋白，广泛存在于真菌、植物和动物中。凝集素结构在动植物领域经过广泛研究。该蛋白质的二级结构富含β-链，并且在表面上具有碳水化合物结合位点。
Other Notes
BS-I是一种四聚体凝集素，由两种类型的亚基A和B组成。存在由不同亚基组成的五种BS-I同工凝集素：BSI-B4、BSI-AB3、BSI-A2B2、BSI-A3B、以及BSI-A4。BSI-B4 是血型B特异的，其对末端α-D-半乳糖残基具有独特的亲和力，而BSI-A4 具有血型A特异性，其对末端N-乙酰基-α-D-半乳糖胺残基具有主要亲和力。
Packaging
基于通过Lowry法测定的蛋白含量进行包装。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
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