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Merck
CN

L3147

Sigma-Aldrich

含琼脂的 LB 肉汤 (Miller)

Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.

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别名:
Luria 琼脂, Miller LB 琼脂
UNSPSC代码:
41106200
eCl@ss:
42040102
NACRES:
NA.85

等级

for molecular biology

质量水平

无菌性

non-sterile

形式

powder

组成

Agar, 15 g/L
NaCl, 10 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

技术

microbiological culture: suitable

pH值(酸碱度)

6.8-7.2(4% solution)

应用

food and beverages
microbiology

储存温度

room temp

适用性

nonselective for Escherichia coli
nonselective for coliforms

一般描述

Miller LB 是一种高度参考的微生物生长培养基,用于培养 大肠埃希菌 。这种营养丰富的微生物肉汤含有多肽、氨基酸、水溶性维生素和碳水化合物。添加琼脂为微生物生长提供固体培养基。

应用

琼脂LB Broth培养基(Miller培养基)用于产生恶臭假单胞菌 KT2440 的单菌落、平板培养大肠杆菌和产生细菌菌株C14和MC8M6的纯菌落。
适用于 大肠杆菌 菌株的非选择性培养,用于克隆、生产DNA质粒和生产重组蛋白。 当加入适当的抗生素时,也适合选择性培养。

特点和优势

含琼脂的 Miller LB 粉末:
  • 使用较大包装尺寸易于放大
  • 一个预算友好的替代预制板
  • 标准配方

制备说明

1.取40g 悬浮于 1L 蒸馏水中。
2.加热至沸腾,同时搅拌溶解。
3.在121°C下高压灭菌15分钟。
4.冷却至 50°C后,分装至无菌培养皿。
制备 Luria-Burrows 培养基:向培养基中加入1 g 葡萄糖,然后按照上述制备说明进行操作。
制备 Luria、Adams和Ting培养基(也称LC琼脂):高压灭菌后,以无菌方式加入 25 ml无菌 0.1 M 氯化钙。

重悬

向 1L 水中加入 40g 粉末。加热至沸腾,同时搅拌以溶解粉末。在 121℃ 下高压灭菌 15 min。在加入抗生素(如需要)之前略微冷却。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What are the differences among the Luria, Lennox and Miller LB formulations?

    LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose.  Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version).  For some applications, even lower salt is required (Luria's low salt version). 

  6. What is the difference among the LB - Miller products?

    LB - Miller is available in many types to suit your needs.  The different product formats include powder and liquid form.  The powder form is also available with agar for easy LB-agar plate preparation.L2542 (LB Miller liquid)L3522 (LB Miller powder)L3147 (LB Miller powder with agar)

  7. Which bacterial culture medium is the best choice for my application?

    Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

  8. Will adding magnesium to the culture medium increase cell density?

    In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities.  You may need to also increase the shaking speed of the incubator. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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McGuigan KG, et al.
Journal of Applied Microbiology, 101(2), 453-463 (2006)
Miller, J.H.
Experiments in Molecular Genetics, 433-433 (1972)
Difco Manual, 239-239 (1998)
Multiple microbial activities for volatile organic compounds reduction by biofiltration
Civilini M.
Journal of the Air & Waste Management Association (1995), 56(7), 922-930 (2006)
Gang Liu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(6), 7718-7732 (2020-04-16)
Liver inflammation is a common extraintestinal manifestation in inflammatory bowel disease (IBD), yet, the mechanisms driving gut-liver axis inflammation remain poorly understood. IBD leads to a breakdown in the integrity of the intestinal barrier causing an increase in portal and

商品

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

基因工程和克隆的发展为研究领域开辟了表达和分离异源蛋白质的许多可能性。技术上巨大进步使得能够大规模表达和分离重组蛋白。

实验方案

General protocols for growth of competent cells in microbial medium.

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

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