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Merck
CN

L3790

Lysozyme 来源于鸡蛋白

10 mg/mL

别名:

粘肽N-乙酰胞壁质水解酶, 胞壁质酶

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关于此项目

化学文摘社编号:
12650-88-3
UNSPSC Code:
12352204
NACRES:
NA.56
EC 号:
3.2.1.17 (BRENDA, IUBMB)
MDL number:
MFCD00131557

主要文件

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产品名称

Lysozyme 来源于鸡蛋白, 10 mg/mL

biological source

chicken egg white

form

liquid

mol wt

single-chain 14.3 kDa

concentration

≥400000 units/mL
10 mg/mL

technique(s)

cell based assay: suitable

suitability

suitable for cell lysis

UniProt accession no.

P00698

application(s)

cell analysis

shipped in

wet ice

storage temp.

−20°C

Quality Level

200

Gene Information

chicken ... LYZ(396218)

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相关类别

Application

适用于细菌 DNA 和蛋白质的纯化。
酶可分解细菌的细胞壁；用于制备原生质体。
采用 CelLytic ™ B 从细菌中提取蛋白质的适用性试验。

Biochem/physiol Actions

溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β（1→4）连接。 革兰氏阳性细胞对这种水解非常敏感，因为它们的细胞壁中的肽聚糖比例很高。革兰氏阴性菌就对这种水解没那么敏感，因为它们有外膜并且肽聚糖的比例较低。然而，这些细胞可能在细胞外膜中螯合金属离子的EDTA存在的情况下水解。

该酶在广泛的pH范围（6.0至9.0）内具有活性。pH值为6.2时，可在更广的离子强度范围（0.02至0.100 M）观察到最大活性，而pH值为9.2时为0.01至0.06 M。
溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β（1→4）连接。革兰氏阳性细胞对这种水解非常敏感，因为它们的细胞壁中的肽聚糖比例很高。

General description

溶酶体是一种存在于蛋清中的酶，它能分解细菌细胞壁，提高提取 DNA 和蛋白质的效率。

Other Notes

酶用缓冲液悬浮后，在pH 8.0、37 °C下，1单位每分钟可释放0.4 μg溶壁微球菌（浊度法检测，600nm）。

Physical form

50% 甘油、25 mM 醋酸钠溶液
即用型溶液：10 mg/mL溶菌酶，溶于含50%甘油的25 mM醋酸钠（pH 4.5）中。

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

低风险生物材料
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000499985
0000499985
0000474874
0000474874
0000432575

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实验方案

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

溶菌酶的酶学测定（EC 3.2.1.17）

该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。

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