L4025
溶壁酶 来源于藤黄节杆菌
lyophilized powder, ≥200 units/mg solid
别名:
细菌溶壁酶, 裂解酶
生物来源
bacterial (Arthrobacter luteus)
质量水平
表单
lyophilized powder
比活
≥200 units/mg solid
技术
cell based assay: suitable
适用性
suitable for cell lysis
应用
diagnostic assay manufacturing
储存温度
−20°C
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应用
藤黄节杆菌(Arthrobacter luteus)溶壁酶可用于重悬细胞以提取高分子量DNA用于PCR（聚合酶链式反应）指纹分析。
藤黄节杆菌（Arthrobacter luteus）裂解酶已用于：
- 作为球状体形成形缓冲液成分，制备酵母核提取物
- 消化样品制剂，用酵母进行β-葡聚糖酶促测定
- 裂解细胞以便RNA分离
生化/生理作用
溶壁酶(Lyticase)常用于真菌研究，特别是用于PCR法（聚合酶链式反应）物种鉴定。可切开糖基之间的β (1→3)和β (1→4)键。
溶壁酶是一种裂解酶，用于通过诱导部分原生质球形成，从酵母细胞中提取DNA。随后再裂解原生质球以释放DNA。酵母细胞的细胞壁可能形成荚膜或耐药孢子，很难破坏，因此溶壁酶成为消化酵母细胞壁的首选方案。溶壁酶含有β-(1→;3)-葡聚糖昆布五糖水解酶以及β-(1→3)-葡聚糖酶、蛋白酶和甘露聚糖酶活性。它适用于多种酵母细胞，例如念珠菌(Candida)、德巴利酵母菌( Debaryomyces)、酿酒酵母(Saccharomyces)、覆膜酵母(Saccharomycopsis)、类酵母酵(Saccharomycodes)、出鞘菌属(Eremothecium)和许旺酵母(Schwanniomyces species)。
溶细胞酶水解聚-β(1→3)-葡萄糖，如酵母细胞壁葡聚糖。
其他说明
一个单位在25°C和pH 7.5条件下，在3ml反应混合物中以酵母悬浮液为底物，每分钟产生0.001的ΔA800 。
仅供研发使用。&不可用于药物、家庭或其他用途。有关危险和安全处理方法的信息，请参阅安全数据说明书。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Naturally occurring novel promoters around pyruvate branch-point for recombinant protein production in Pichia pastoris (Komagataella phaffii): pyruvate decarboxylase-and pyruvate kinase-promoters
Massahi A and Calik P
Biochemical Engineering Journal, 138, 111-120 (2018)
M Groenewald et al.
Persoonia, 21, 17-27 (2008-12-01)
The type species of the genus Debaryomyces, Debaryomyces hansenii, is a highly heterogeneous species. It has been isolated from a large diversity of natural sources including fruit, air, water, soil, but most frequently from processed food products. The species delineation
A protein transformation protocol for introducing yeast prion particles into yeast
Tanaka M, et al.
Methods in Enzymology, 470, 681-693 (2010)
Lisbeth Rosager Poulsen et al.
The Plant cell, 20(3), 658-676 (2008-03-18)
Vesicle budding in eukaryotes depends on the activity of lipid translocases (P(4)-ATPases) that have been implicated in generating lipid asymmetry between the two leaflets of the membrane and in inducing membrane curvature. We show that Aminophospholipid ATPase3 (ALA3), a member
Jonathan G Van Vranken et al.
Molecular cell, 71(4), 567-580 (2018-08-18)
The electron transport chain (ETC) is an important participant in cellular energy conversion, but its biogenesis presents the cell with numerous challenges. To address these complexities, the cell utilizes ETC assembly factors, which include the LYR protein family. Each member
实验方案
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
本操作程序可用于使用面包酵母作为底物测定溶壁酶的活性。
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