Merck
CN

L4631

Sigma-Aldrich

Lysozyme 来源于鸡蛋白

For use as a marker in SDS-PAGE

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别名:
粘肽N-乙酰胞壁质水解酶, 胞壁质酶
CAS号:
EC 号:
MDL编号:
NACRES:
NA.54

生物来源

chicken egg white

质量水平

形式

powder

分子量

single-chain 14.3 kDa

包装

vial of 25 mg

UniProt登记号

储存温度

2-8°C

基因信息

chicken ... LYZ(396218)

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应用

酶可分解细菌的细胞壁;用于制备原生质体。

生化/生理作用

溶菌酶可水解肽聚糖中N-乙酰胞壁酸与N-乙酰-D-氨基葡萄糖残基之间以及壳糊精中N-乙酰-D-氨基葡萄糖残基之间的β(1→4)连接。 革兰氏阳性细胞对这种水解非常敏感,因为它们的细胞壁中的肽聚糖比例很高。革兰氏阴性菌就对这种水解没那么敏感,因为它们有外膜并且肽聚糖的比例较低。然而,这些细胞可能在细胞外膜中螯合金属离子的EDTA存在的情况下水解。

该酶在广泛的pH范围(6.0至9.0)内具有活性。pH值为6.2时,可在更广的离子强度范围(0.02至0.100 M)观察到最大活性,而pH值为9.2时为0.01至0.06 M。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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High-resolution inelastic X-ray scattering was used to investigate the collective vibrational excitations in hydrated lysozyme powders as a function of hydration level and temperature. It is found that the samples with strong enzymatic function are "soft", in the sense that

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