Anti-LSD1 (AOF2) (N-terminal) antibody produced in rabbit

Lysine specific demethylase 1 (LSD1) also called Amine oxidase flavin-containing domain 2 (AOF2) is a nuclear protein containing a Swi3p, Rsc8p and Moira (SWIRM) domain, a flavin adenine dinucleotide (FAD)-binding motif, and an amine oxidase domain. LSD1 gene is mapped to human chromosome 1p36.12.

synthetic peptide corresponding to amino acids 123-139 of human LSD1 (AOF2) (isoform b), conjugated to KLH via a C-terminal added cysteine residue. The immunizing sequence is present also in isoform a. This sequence is conserved in human, rat, and mouse.

Anti-LSD1 (AOF2) (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Anti-LSD1 (AOF2)(N-terminal) specifically recognizes human LSD1 (AOF2).

Lysine specific demethylase 1 (LSD1) is a component of several histone deacetylase complexes that function through modifying chromatin structure to repress transcription. Methylation of lysine can lead either to gene silencing or activation depending on the specific lysine residue that is methylated. LSD1 action can signal either activation or repression of transcription. The demethylation of histone H3 Lys4 by LSD1 leads to transcriptional repression of target genes, while demethylation of histone H3 at Lys9 leads to de-repression of androgen receptor target genes. The activity of LSD1 is regulated by its associated factors. Corepressor of RE1 silencing transcription factor/neural-restrictive silencing factor (REST), a transcriptional corepressor, promotes demethylation by enhancing the association of LSD1 with the nucleosomes and also protects LSD1 from proteasomal degradation.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

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