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Merck
CN

L4895

来自 普通小麦 的凝集素(小麦)

FITC conjugate, lyophilized powder

别名:

WGA, Wheat germ agglutinin

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关于此项目

NACRES:
NA.32
UNSPSC Code:
12352202
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产品名称

来自 普通小麦 的凝集素(小麦), FITC conjugate, lyophilized powder

conjugate

FITC conjugate

form

lyophilized powder

potency

<50 μg/mL agglutination activity

composition

Protein, ~50% Lowry

extent of labeling

~2 mol FITC per mol protein

storage temp.

2-8°C

Quality Level

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Application

Triticum vulgaris(小麦)来源凝集素已用于:
  • 处理转染细胞,以进行免疫荧光分析
  • 立体定位注射到小鼠体内,以进行神经元示踪
  • 免疫组化,以分析心肌细胞

用于荧光检测含有 β(1→4)-N-乙酰基-D葡糖胺的糖蛋白

Biochem/physiol Actions

WGA 不具有血型特异性,但具有与 N-乙酰基-β-D-葡糖胺残基和 N-乙酰基-β-D-葡糖胺低聚物的亲和性。WGA 不含蛋白结合糖。
凝集素在糖缀合物鉴定、成像和靶向过程中发挥着关键作用。它可用于芯片分析,实现有效的糖组图谱分析。在植物和真菌体内,凝集素可防御病原体/寄生物。凝集素在宿主识别和组织粘附中同样发挥主要作用。

General description

凝集素是碳水化合物结合蛋白。它们无处不在,常见于真菌、植物和动物体内。该蛋白质的二级结构富含β-链,并且在表面上具有碳水化合物结合位点。

Packaging

基于蛋白含量进行包装。

Physical form

含有磷酸盐缓冲盐

Preparation Note

由亲和纯化凝集素制备偶联物。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品
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历史批次信息供参考:

分析证书(COA)

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Yihua Bei et al.
Journal of cellular and molecular medicine, 21(8), 1648-1655 (2017-03-18)
The adult heart retains a limited ability to regenerate in response to injury. Although exercise can reduce cardiac ischaemia/reperfusion (I/R) injury, the relative contribution of cardiac cell proliferation including newly formed cardiomyocytes remains unclear. A 4-week swimming murine model was
Robert H Oakley et al.
Science signaling, 12(577) (2019-04-18)
Stress is increasingly associated with heart dysfunction and is linked to higher mortality rates in patients with cardiometabolic disease. Glucocorticoids are primary stress hormones that regulate homeostasis through two nuclear receptors, the glucocorticoid receptor (GR) and mineralocorticoid receptor (MR), both
Rui Guo et al.
Scientific reports, 7(1), 8703-8703 (2017-08-20)
Diabetes is a major risk factor for cardiovascular disease and the lysosomal cysteine protease cathepsin K plays a critical role in cardiac pathophysiology. To expand upon our previous findings, we tested the hypothesis that, knockout of cathepsin K protects against
Novel Chemical Suppressors of Long QT Syndrome Identified by an In Vivo Functional Screen
Peal DS, et al.
Circulation (2011)
Midbrain dopamine neurons associated with reward processing innervate the neurogenic subventricular zone
Lennington JB, et al.
The Journal of Neuroscience, 31(37), 13078-13087 (2011)

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