产品名称
来自 普通小麦 的凝集素（小麦）, biotin conjugate, lyophilized powder
conjugate
biotin conjugate
form
lyophilized powder
composition
Protein, ≥80% E1%/280
extent of labeling
2-4 mol biotin per mol protein
storage temp.
2-8°C
Quality Level
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Physical form
含有柠檬酸钠
Preparation Note
由亲和纯化凝集素制备偶联物。
Legal Information
Dynabeads is a registered trademark of Invitrogen Dynal AS Corp
Application
来自Triticum vulgaris （小麦）的凝集素已被用于：
- 丝虫寄生虫的子宫内阶段的样品的凝集素结合测定中
- 小鼠心肌细胞的组织学研究中
- 与Dynabeads一起形成珠子-生物素-WGA复合物® M-280链霉抗生物素蛋白磁珠，用于对来自水样中的弓形虫卵母细胞中的凝集素进行磁性分离
Biochem/physiol Actions
WGA 不具有血型特异性，但具有与 N-乙酰基-β-D-葡糖胺残基和 N-乙酰基-β-D-葡糖胺低聚物的亲和性。WGA 不含蛋白结合糖。
General description
来自Triticum vulgaris的小麦胚芽凝集素（WGA）以同型二聚体形式存在，并且具有多个N-乙酰葡糖胺（GlcNAc）结合位点。它对蛋白质变性剂具有抗性，可用于检测 O-GlcNAc蛋白。 WGA与N-乙醇酰神经氨酸（Neu5Ac）结合可调节促炎免疫应答。
Packaging
包装尺寸基于蛋白质含量。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
此项目有
WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins
Kubota Y, et al.
Testing, 12(7), e0180714-e0180714 (2017)
Candida Fagundes Teixeira et al.
PLoS neglected tropical diseases, 1(2), e73-e73 (2007-12-07)
Diagnosis of intestinal schistosomiasis in low endemic areas is a problem because often control measures have reduced egg burdens in feces to below the detection limits of classical coproparasitological methods. Evaluation of molecular methods is hindered by the absence of
R E Morris et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 40(5), 711-721 (1992-05-01)
We demonstrate here that the intracellular routing of biotinylated ligands was not affected by the attachment of streptavidin gold colloids so long as the electron-dense marker was added after the biotinyl ligand-receptor interaction had occurred. The binding, internalization, and intracellular
Evaluation of bi-ventricular coronary flow patterns using high-frequency ultrasound in mice with transverse aortic constriction
Wu J, et al.
Ultrasound in Medicine & Biology, 39(11), 2053-2065 (2013)
The dietary intake of wheat and other cereal grains and their role in inflammation
De PK and Pruimboom L
Nutrients, 5(3), 771-787 (2013)
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