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Merck
CN

L5142

来自 普通小麦 的凝集素(小麦)

biotin conjugate, lyophilized powder

别名:

WGA, Wheat germ agglutinin

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关于此项目

UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
34058011
Form:
lyophilized powder
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conjugate

biotin conjugate

Quality Level

form

lyophilized powder

composition

Protein, ≥80% E1%/280

extent of labeling

2-4 mol biotin per mol protein

storage temp.

2-8°C

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General description

来自Triticum vulgaris的小麦胚芽凝集素(WGA)以同型二聚体形式存在,并且具有多个N-乙酰葡糖胺(GlcNAc)结合位点。它对蛋白质变性剂具有抗性,可用于检测 O-GlcNAc蛋白。 WGA与N-乙醇酰神经氨酸(Neu5Ac)结合可调节促炎免疫应答。

Application

来自Triticum vulgaris (小麦)的凝集素已被用于:
  • 丝虫寄生虫的子宫内阶段的样品的凝集素结合测定中
  • 小鼠心肌细胞的组织学研究中
  • 与Dynabeads一起形成珠子-生物素-WGA复合物® M-280链霉抗生物素蛋白磁珠,用于对来自水样中的弓形虫卵母细胞中的凝集素进行磁性分离

Biochem/physiol Actions

WGA 不具有血型特异性,但具有与 N-乙酰基-β-D-葡糖胺残基和 N-乙酰基-β-D-葡糖胺低聚物的亲和性。WGA 不含蛋白结合糖。

Packaging

包装尺寸基于蛋白质含量。

Physical form

含有柠檬酸钠

Preparation Note

由亲和纯化凝集素制备偶联物。

Legal Information

Dynabeads is a registered trademark of Invitrogen Dynal AS Corp

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

低风险生物材料

此项目有


历史批次信息供参考:

分析证书(COA)

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A flow cytometry based method for studying embryogenesis and immune reactivity to embryogenic stages in filarial parasites
Sahu BR, et al.
Filaria Journal, 4(1), 11-11 (2005)
The dietary intake of wheat and other cereal grains and their role in inflammation
De PK and Pruimboom L
Nutrients, 5(3), 771-787 (2013)
Evaluation of bi-ventricular coronary flow patterns using high-frequency ultrasound in mice with transverse aortic constriction
Wu J, et al.
Ultrasound in Medicine & Biology, 39(11), 2053-2065 (2013)
WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins
Kubota Y, et al.
Testing, 12(7), e0180714-e0180714 (2017)
Jemere Bekele Harito et al.
Water research, 127, 68-76 (2017-10-17)
Proof-of-principle of lectin-magnetic separation (LMS) for isolating Toxoplasma oocysts (pre-treated with 0.5% acidified pepsin (AP)) from water for subsequent detection by microscopy or molecular methods has been shown. However, application of this technique in the routine water-analysis laboratory requires that

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全球贸易项目编号

货号GTIN
L5142-5MG04061833263396
L5142-1MG04061833957912

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